Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies
Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies
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DOI：
10.1038/ncb1265
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发表时间：
2005-06-01
影响因子：
21.3
通讯作者：
Blau, HM
中科院分区：
文献类型：
--
作者：
Sen, GL;Blau, HM
RNA干扰(RNAi)是消除mRNAs的重要手段，但RNA诱导的沉默复合体(RISC)在细胞内的定位尚不清楚。我们在这里表明，作为RISC的关键成分，ArgAerte 2不是随机分布的，而是集中在被称为细胞质小体的mRNA衰变中心。小干扰RNA或其靶向RNA的存在或不存在不会改变ArgAerte2在衰变中心的定位。然而，RNA对于细胞质小体的完整性是必需的，因为核糖核酸酶消除了ArgAerte2的定位。此外，Argavite家族的另一成员ArgAerte1主要集中在细胞质中。这些结果为研究RNAi的作用机制提供了新的思路。
RNA interference (RNAi) is an important means of eliminating mRNAs, but the intracellular location of RNA-induced silencing complex (RISC) remains unknown. We show here that Argonaute 2, a key component of RISC, is not randomly distributed but concentrates in mRNA decay centres that are known as cytoplasmic bodies. The localization of Argonaute 2 in decay centres is not altered by the presence or absence of small interfering RNAs or their targeted mRNAs. However, RNA is required for the integrity of cytoplasmic bodies because RNase eliminates Argonaute 2 localization. In addition, Argonaute 1, another Argonaute family member, is concentrated in cytoplasmic bodies. These results provide new insight into the mechanism of RNAi function.