Effects of Ag Nanoparticles on Growth and Fat Body Proteins in Silkworms (Bombyx mori).
Effects of Ag Nanoparticles on Growth and Fat Body Proteins in Silkworms (Bombyx mori).
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纳米银颗粒对家蚕生长和脂肪体蛋白的影响
DOI：
10.1007/s12011-017-1001-7
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发表时间：
2017-12
影响因子：
3.9
通讯作者：
Chen K
中科院分区：
文献类型：
--
作者：
Meng X;Abdlli N;Wang N;Lü P;Nie Z;Dong X;Lu S;Chen K
银纳米颗粒(AgNPs)是一种广泛使用的非抗生素、抗菌材料，已显示出对哺乳动物的毒性和其他潜在的有害影响。然而，AgNPs对昆虫的危害尚不清楚。在这里，我们研究了AgNPs对模型无脊椎动物家蚕的影响。用不同浓度的AgNPs饲喂家蚕幼虫后，观察其对B的影响。胎体重量、存活率和蛋白质组学差异。结果表明，低浓度(400 mg/L)的AgNPs对B的生长和蚕茧量有促进作用。森喜朗。尽管高浓度(≥800m g/L)的AgNPs也改善了B。早熟的时候，它们导致了家蚕的死亡。对脂肪体蛋白质组差异的分析发现，脂肪体内蛋白质点有13个显著差异，其中9个显著下调，4个显著上调。逆转录-聚合酶链式反应结果显示，在AgNP浓度为1600 mg/L时，7种蛋白质的表达水平与其对应基因的转录水平相似。我们的结果表明，AgNPs通过调节相关的蛋白质代谢和代谢途径，降低对氧化应激的抵抗力，影响细胞凋亡，诱导细胞坏死。森喜朗。
Ag nanoparticles (AgNPs), a widely used non-antibiotic, antibacterial material, have shown toxic and other potentially harmful effects in mammals. However, the deleterious effects of AgNPs on insects are still unknown. Here, we studied the effects of AgNPs on the model invertebrate organismBombyx mori. After feeding silkworm larvae different concentrations of AgNPs, we evaluated the changes ofB. moribody weights, survival rates, and proteomic differences. The results showed that low concentrations (<400 mg/L) of AgNPs promoted the growth and cocoon weights ofB. mori. Although high concentrations (≥800 mg/L) of AgNPs also improvedB. morigrowth, they resulted in silkworm death. An analysis of fat body proteomic differences revealed 13 significant differences in fat body protein spots, nine of which exhibited significantly downregulated expression, while four showed significantly upregulated expression. Reverse transcription–polymerase chain reaction results showed that at an AgNP concentration of 1600 mg/L, the expression levels of seven proteins were similar to the transcription levels of their corresponding genes. Our results suggest that AgNPs lowered the resistance to oxidative stress, affected cell apoptosis, and induced cell necrosis by regulating related protein metabolism and metabolic pathways inB. mori.
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DOI：
10.1016/j.cbpb.2010.12.001
发表时间：
2011-03-01
影响因子：
2.2
作者：
Kang, Lequn;Shi, Haifeng;Chen, Keping
通讯作者：
Chen, Keping
影响因子：
23.8
作者：
Arrese EL;Soulages JL
通讯作者：
Soulages JL
影响因子：
17.1
作者：
Liu, Jingyu;Wang, Zhongying;Liu, Frances D.;Kane, Agnes B.;Hurt, Robert H.
通讯作者：
Hurt, Robert H.
影响因子：
2.2
作者：
Afrasiabi, Zahra;Popham, Holly J. R.;Upendran, Anandhi
通讯作者：
Upendran, Anandhi
影响因子：
3.3
作者：
Licker, Virginie;Cote, Melanie;Burkhard, Pierre R.
通讯作者：
Burkhard, Pierre R.