Stimulation of proliferation, differentiation, and function of human cells by primate interleukin 3.
Stimulation of proliferation, differentiation, and function of human cells by primate interleukin 3.
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DOI：
10.1073/pnas.84.9.2761
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发表时间：
1987-05
影响因子：
11.1
通讯作者：
A. Lopez;L. To;Yuh Cheng Yang;J. Gamble;M. Shannon;G. Burns;P. Dyson;C. Juttner;S. Clark;M. Vadas
中科院分区：
文献类型：
--
作者：
A. Lopez;L. To;Yuh Cheng Yang;J. Gamble;M. Shannon;G. Burns;P. Dyson;C. Juttner;S. Clark;M. Vadas
研究发现克隆长臂猿白细胞介素 3 (gIL-3) 可刺激人骨髓细胞的增殖和分化，在半固体琼脂中产生第 14 天粒细胞、巨噬细胞、粒细胞-巨噬细胞和嗜酸性粒细胞集落。在正常人血浆存在的情况下，gIL-3 刺激巨核细胞。在甲基纤维素培养物中，它在促红细胞生成素存在的情况下刺激红细胞集落，但在促红细胞生成素不存在的情况下则不会。当使用成熟的人白细胞时，gIL-3刺激纯化的成熟嗜酸性粒细胞的功能，通过杀死抗体包被的靶细胞的能力来测量，产生超氧阴离子，并以类似于重组人粒细胞-巨噬细胞集落刺激因子的方式吞噬调理的酵母颗粒。相比之下，gIL-3 没有显着刺激任何测试的中性粒细胞功能，而人重组粒细胞-巨噬细胞集落刺激因子在这些测定中具有活性。因此，在对人类造血细胞有活性的细胞因子中，gIL-3 具有一组独特的功能，并且可以预测人类分子的作用范围。
Cloned gibbon interleukin 3 (gIL-3) was found to stimulate the proliferation and differentiation of human bone marrow cells to produce day-14 granulocyte, macrophage, granulocyte-macrophage, and eosinophil colonies in semisolid agar. In the presence of normal human plasma, gIL-3 stimulated megakaryocytes. In methylcellulose cultures, it stimulated erythroid colonies in the presence, but not in the absence, of erythropoietin. When mature human leukocytes were used, gIL-3 stimulated the function of purified mature eosinophils as measured by the capacity to kill antibody-coated target cells, to produce superoxide anions, and to phagocytize opsonized yeast particles in a manner similar to recombinant human granulocyte-macrophage colony-stimulating factor. In contrast, gIL-3 did not significantly stimulate any of the neutrophil functions tested, whereas human recombinant granulocyte-macrophage colony-stimulating factor was active in these assays. Among cytokines that are active on human hematopoietic cells, gIL-3 thus has a distinct set of functions and may predict the range of actions of the human molecule.