Determination of Endogenous Bufalin in Serum of Patients With Hepatocellular Carcinoma Based on HPLC-MS/MS
Determination of Endogenous Bufalin in Serum of Patients With Hepatocellular Carcinoma Based on HPLC-MS/MS
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基于HPLC-MS/MS法测定肝细胞癌患者血清中内源性蟾蜍灵
DOI：
10.3389/fonc.2019.01572
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发表时间：
2020-01-23
影响因子：
4.7
通讯作者：
Su, Yonghua
中科院分区：
文献类型：
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作者：
Han, Mengfei;Yang, Geliang;Su, Yonghua
蟾毒灵是一种强心类固醇，也是中药蟾酥的主要活性成分。它对许多恶性肿瘤具有显著的抗肿瘤活性，包括肝细胞癌（HCC）。先前的研究表明，人体内含有一种内源性蟾蜍灵样物质。本研究旨在通过高效液相色谱-串联质谱（HPLC-MS/MS）技术，确认内源性蟾蜍灵样物质是否为蟾蜍灵，并进一步检测肝癌组与对照组内源性蟾蜍灵浓度的差异。实验结果证实了蟾蜍内源性蟾蜍灵样物质为蟾蜍灵。共收集了227份血清样本：54份来自HCC患者，173份来自健康志愿者，构成对照组。实验组和对照组血清中均含有蟾毒灵，表明蟾毒灵确实是一种内源性物质。蟾蜍灵在肝癌患者中的浓度为1.3nM，在正常人中为5.7nM（P < 0.0001）。这些结果表明，人体内含有蟾蜍灵，它可能与肝癌的发病率呈负相关。
Bufalin is a cardiotonic steroid and a key active ingredient of the Chinese medicine ChanSu. It has significant anti-tumor activity against many malignancies, including hepatocellular carcinoma (HCC). Previous studies have shown that human bodies contain an endogenous bufalin-like substance. This study aimed to confirm whether the endogenous bufalin-like substances is bufalin and further detect the differences between HCC and control groups of endogenous bufalin concentration by the high-performance liquid chromatography coupled tandem mass spectrometry (HPLC-MS/MS). The results confirmed the endogenous bufalin-like substance is bufalin. Totally, 227 serum samples were collected: 54 from HCC patients and 173 from healthy volunteers constituting a control group. Both the test group and the control group contained bufalin in serum, revealing that bufalin is indeed an endogenous substance. The bufalin concentration was 1.3 nM in HCC patients and 5.7 nM in normal people (P < 0.0001). These results indicate that human bodies contain endogenous bufalin, and it may be negatively correlated with the incidence of HCC.