Green synthesis of silver nanoparticles by Nigella sativa extract alleviates diabetic neuropathy through anti-inflammatory and antioxidant effects
Green synthesis of silver nanoparticles by Nigella sativa extract alleviates diabetic neuropathy through anti-inflammatory and antioxidant effects
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DOI：
10.1016/j.sjbs.2020.05.005
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发表时间：
2020-09-01
影响因子：
4.4
通讯作者：
Hamza, Amal
中科院分区：
文献类型：
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作者：
Alkhalaf, Maha I.;Hussein, Rasha H.;Hamza, Amal
绿色合成银纳米粒子引起了科学家们的极大兴趣。鉴于这些数据，我们进行了这项研究，以确定使用黑种草提取物的绿色合成的银纳米颗粒在实验诱导的糖尿病神经病变中的改善效果。本研究选用成年雄性白化病大鼠50只，随机分为5组，第1组为健康对照组，第2组为糖尿病神经病变诱导组，第3-6组为糖尿病神经病变诱导组，分别给予银纳米颗粒、黑种草提取物和黑种草提取物绿色合成银纳米颗粒治疗。评估了包括糖尿病、炎症和抗氧化生物标志物在内的生化参数。还进行了脑组织病理学检查。结果显示，与健康对照组相比，糖尿病神经病变诱导组的葡萄糖、AGE、醛糖还原酶显著升高，胰岛素降低。糖尿病神经病变组炎症指标明显升高。在同一组中观察到氧化状态的显着变化。此外，观察到硝基酪氨酸水平显著下降。关于基因表达，我们发现与健康对照组相比，糖尿病神经病变组的脑TKr A显著下调，伴随着神经生长因子的上调。糖尿病神经病变的几种治疗方法显着改善了所有研究的生物标志物。本研究所取得的结果在很大程度上依赖于组织学发现。因此，可以确定绿色合成的银纳米颗粒与黑种草提取物的组合可以通过其抗糖尿病、抗炎和抗氧化作用而成为对抗炎症和氧化应激的新的神经保护剂，所述炎症和氧化应激表征糖尿病神经病变。(C)2020年，任作家。由Elsevier B. V.代表沙特国王大学出版。
Green synthesis of silver nanoparticles has gained great interest among scientists. In view of this data, we conducted this study to identify the ameliorative effect of green synthesis of silver nanoparticles using Nigella sativa extract in diabetic neuropathy induced experimentally. In this study, 50 adult male albino rats were used and they were randomly divided into five groups; the first group was the healthy control group, the second group were the diabetic neuropathy diabetic neuropathy induced, Groups (3-6) diabetic neuropathy induced group and treated with silver nanoparticles, Nigella sativa extract and green synthesized silver nanoparticles using Nigella sativa extract respectively. Biochemical parameters including diabetic, inflammatory and antioxidant biomarkers were evaluated. Brain histopathology was also performed. Results revealed substantial rise in glucose, AGE, aldose reductase with insulin reduction in diabetic neuropathy induced group as compared to healthy control. Also, inflammatory markers increased significantly in diabetic neuropathy induced group. A remarkable change in oxidative status was observed in the same group. Furthermore, significant decline in nitrotyrosin level was observed. Regarding gene expression, we found significant down regulation in brain TKr A accompanied by upregulation of nerve growth factor in diabetic neuropathy group comparing with healthy control. Several treatments for diabetic neuropathy remarkably ameliorate all the investigated biomarkers. Histological findings are greatly relied on for the results achieved in this study. Therefore, it can be established that green synthesis of silver nanoparticles in combination with Nigella sativa extract could be a newly neuroprotective agents against inflammation and oxidative stress characterizing diabetic neuropathy through their antidiabetic, anti-inflammatory and anti-oxidants effects. (C) 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud University.