Macrophage foam cell formation is augmented in serum from patients with diabetic angiopathy
Macrophage foam cell formation is augmented in serum from patients with diabetic angiopathy
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DOI：
10.1016/j.diabres.2009.10.011
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发表时间：
2010-01-01
影响因子：
5.1
通讯作者：
Asano, Tomoichiro
中科院分区：
文献类型：
--
作者：
Cui, Xinglong;Kushiyama, Akifumi;Asano, Tomoichiro
巨噬细胞分化为分泌精氨酸的泡沫细胞在糖尿病血管病变的发展中起着关键作用。据报道，J774.1是一种鼠巨噬细胞系，当与氧化LDL、富含ApoE的VLDL或WHHLMI（心肌梗死倾向性Watanabe遗传性高血脂症）兔血清孵育时，其分化为泡沫细胞。在这项研究中，将来自2型糖尿病患者的血清样品加入到具有J774.1细胞的培养基中，并通过测量脂质积累来定量泡沫细胞诱导的程度。这些值相对于正常和WHHLMI兔血清的活性分别计算为0%和100%，并称为MMI（巨噬细胞成熟指数）。这些MMI值反映了细胞内脂质，包括胆固醇酯测定GC/MS。统计分析显示MMI与血清甘油三酯，糖尿病视网膜病变，肾病和肥胖症的状态呈正相关和独立，但与α-葡萄糖苷酶抑制剂或噻唑烷二酮类药物的管理呈负相关。综上所述，我们的研究结果表明，这种新的检测方法可能适用于快速进展性血管病变的风险患者的识别。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
The differentiation of macrophages into cytokine-secreting foam cells plays a critical role in the development of diabetic angiopathy. J774.1, a murine macrophage cell line, reportedly differentiates into foam cells when incubated with oxidized LDL, ApoE-rich VLDL or WHHLMI (myocardial infarction-prone Watanabe heritable hyperlipidemic) rabbit serum. In this study, serum samples from Type 2 diabetic patients were added to the medium with J774.1 cells and the degree of foam cell induction was quantified by measuring lipid accumulation. These values were calculated relative to the activities of normal and WHHLMI rabbit sera as 0% and 100%, respectively, and termed the MMI (Macrophage Maturation Index). These MMI values reflected intracellular lipids, including cholesteryl ester assayed by GC/MS. Statistical analysis revealed MMI to correlate positively and independently with serum triglycerides, the state of diabetic retinopathy, nephropathy and obesity, but negatively with administration of alpha-glucosidase inhibitors or thiazolidinediones. Taken together, our results suggest that this novel assay may be applicable to the identification of patients at risk for rapidly progressive angiopathic disorders. (C) 2009 Elsevier Ireland Ltd. All rights reserved.