Single Dose Administration of L-Carnitine Improves Antioxidant Activities in Healthy Subjects
Single Dose Administration of L-Carnitine Improves Antioxidant Activities in Healthy Subjects
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DOI：
10.1620/tjem.224.209
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发表时间：
2011-07-01
影响因子：
2.2
通讯作者：
Han, Zhi-wu
中科院分区：
文献类型：
--
作者：
Cao, Yu;Qu, Hai-jun;Han, Zhi-wu
左旋肉碱已被用作治疗心血管或肝脏疾病的补充剂。然而，关于左旋肉碱对健康人体抗氧化能力的影响的信息很少。本研究旨在探讨血浆左旋肉碱浓度与抗氧化活性之间的相关性。 12 名健康受试者以单剂量口服液体左旋肉碱（2.0 克）。通过HPLC检测L-肉碱的血浆浓度。左旋肉碱的基线浓度为39.14+/-5.65μmol/L。单次口服给药后，最大血药浓度（C-max）和曲线下面积（AUC（0-无穷大））分别为84.7±25.2μmol/L和2,676.4±708.3μmol/L.h。半衰期和达到 C-max 所需的时间分别为 60.3 +/- 15.0 分钟和 3.4 +/- 0.46 小时。在左旋肉碱给药后的前3.5小时内，超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶和总抗氧化能力（T-AOC）的血浆浓度逐渐增加。 SOD、GSH-Px、过氧化氢酶和T-AOC的血浆浓度在24小时内恢复到基线水平。左旋肉碱浓度与SOD（r = 0.992，P < 0.01）、GSH-Px（r = 0.932，P < 0.01）、过氧化氢酶（r = 0.972，P < 0.01）或T-AOC（r = 0.934，P < 0.01）抗氧化指数呈正相关。总之，左旋肉碱可提高健康受试者的抗氧化酶活性和总抗氧化能力。它可能可用作涉及过度氧化应激的慢性疾病的补充疗法。
L-carnitine has been used as a supplement to treat cardiovascular or liver disease. However, there has been little information about the effect of L-carnitine on anti-oxidation capability in healthy human subjects. This study was designed to investigate the correlation between plasma L-carnitine concentration and antioxidant activity. Liquid L-carnitine (2.0 g) was administered orally as a single dose in 12 healthy subjects. Plasma concentration of L-carnitine was detected by HPLC. The baseline concentration of L-carnitine was 39.14 +/- 5.65 mu mol/L. After single oral administration, the maximum plasma concentration (C-max) and area under the curve (AUC(0-infinity)) were 84.7 +/- 25.2 mu mol/L and 2,676.4 +/- 708.3 mu mol/L.h, respectively. The half-life and the time required to reach the C-max was 60.3 +/- 15.0 min and 3.4 +/- 0.46 h, respectively. There was a gradual increase in plasma concentrations of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase and total antioxidative capacity (T-AOC) in the first 3.5 h following L-carnitine administration. The plasma concentrations of SOD, GSH-Px, catalase and T-AOC returned to baseline levels within 24 h. A positive correlation was found between L-carnitine concentration and the antioxidant index of SOD (r = 0.992, P < 0.01), GSH-Px (r = 0.932, P < 0.01), catalase (r = 0.972, P < 0.01) or T-AOC (r = 0.934, P < 0.01). In conclusion, L-carnitine increases activities of antioxidant enzymes and the total antioxidant capacity in healthy subjects. It may be useful as a supplementary therapy for chronic illnesses involving excessive oxidative stress.