Improving the Retention Rate of Fat Grafts in Recipient Areas via Botulinum Toxin A Treatment
Improving the Retention Rate of Fat Grafts in Recipient Areas via Botulinum Toxin A Treatment
复制标题
通过 A 型肉毒杆菌毒素治疗提高受区脂肪移植物的保留率
DOI：
10.1093/asj/sjz073
复制
发表时间：
2019
影响因子：
2.9
通讯作者：
Yi Chenggang
中科院分区：
文献类型：
--
作者：
Shi Nian;Su Yingjun;Guo Shuzhong;Zhang Zhaoxiang;Qiu Lihong;Yi Chenggang
背景提高移植脂肪的留存率是目前非常关注的问题。部分固定，血管生成，和脂肪组织来源的干细胞，所有被证明是由肉毒杆菌毒素A（BTX-A）的影响，是显着的脂肪移植retention.ObjectivesThe authors试图确定BTX-A对脂肪grafts.MethodsOur研究的影响包括12 Sprague道利大鼠和每只大鼠的后肢被随机指定为BTX-A侧和对照侧。我们将0.2 mL BTX-A处理的脂肪注射到股四头肌和BTX-A侧的皮下空间中。这也是对对照侧进行的，但使用未经处理的脂肪。术后1周行受体肌肉神经肌电图检查。术后12周处死大鼠，观察各组大鼠脂肪留存率、脂肪组织学特征、血管密度和成熟脂肪细胞密度。对于肌内注射脂肪，BTX-A侧比对照侧具有更好的保留率和组织学特征以及更高的血管和成熟脂肪细胞密度。对于皮下注射的脂肪，BTX-A侧有更好的组织学特征和更高的密度的血管和成熟的脂肪细胞比对照侧，但保留率之间没有显着差异。BTX-A可改善血管密度、成熟脂肪细胞密度、脂肪移植物的组织学特性及脂肪移植物的留存率。
BackgroundImproving the retention rate of transplanted fat is, currently, of great concern. Partial immobilization, angiogenesis, and adipose tissue-derived stem cells, all proven to be influenced by botulinum toxin A (BTX-A), are significant in fat graft retention.ObjectivesThe authors sought to determine the impact of BTX-A on fat grafts.MethodsOur study included 12 Sprague Dawley rats and each rat’s hind limbs were randomly designated as the BTX-A side and control side. We injected 0.2 mL of BTX-A-treated fat into the quadriceps femoris and subcutaneous space of the BTX-A sides. This was also done for the control sides but with untreated fat. We performed electroneuromyography of recipient muscles at 1 week post-operation. The rats were euthanized at 12 weeks post-operation and we observed the fat retention rate, the fat’s histologic characteristics, and the density of vessels and mature adipocytes.ResultsThe amplitudes of electroneuromyography were smaller for the BTX-A sides than the control sides. For intramuscularly injected fat, the BTX-A sides had better retention rates and histologic characteristics and a higher density of vessels and mature adipocytes than the control sides. For subcutaneously injected fat, the BTX-A sides had better histologic characteristics and a higher density of vessels and mature adipocytes than the control sides, but the retention rates were not significantly different between the 2 sides.ConclusionsInjecting BTX-A-treated fat grafts can immobilize the surrounding muscles. BTX-A can improve the density of vessels and mature adipocytes, histologic characteristics of fat grafts, and retention rate of fat grafts transplanted into muscles.