CD OF PROLINE-RICH POLYPEPTIDES - APPLICATION TO THE STUDY OF THE REPETITIVE DOMAIN OF MAIZE GLUTELIN-2
CD OF PROLINE-RICH POLYPEPTIDES - APPLICATION TO THE STUDY OF THE REPETITIVE DOMAIN OF MAIZE GLUTELIN-2
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DOI：
10.1002/bip.360330704
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发表时间：
1993-07-01
期刊：
影响因子：
2.9
通讯作者：
GIRALT, E
中科院分区：
文献类型：
--
作者：
RABANAL, F;LUDEVID, MD;GIRALT, E
本文综述了富含脯氨酸多肽的圆二色谱。首先，结构特征，理论CD研究，和生物相关性的聚脯氨酸II结构在这样的肽进行了讨论。第二，描述了属于玉米谷蛋白-2的重复结构域的肽H-（Val-His-Leu-Pro-Pro-Pro）n-OH（n = 3，5，8）的CD研究。这一系列的肽显示了聚脯氨酸II结构在水中（5 ℃，pH 5）的CD功能。此外，它表明，增加量的聚阴离子分子肝素的加入迫使位移的构象平衡，这些肽向更高比例的聚脯氨酸II结构。相反，当温度升高时，这种结构逐渐消失，导致更无序的构象。
An overview of CD of proline-rich peptides is reported. First, structural characteristics, theoretical CD studies, and the biological relevance of polyproline II structure in such peptides are discussed. Second, a CD study of peptides belonging to the repetitive domain of maize glutelin-2, H- (Val-His-Leu-Pro-Pro-Pro)n-OH (n = 3, 5, 8), is described. This series of peptides displayed the CD features of polyproline II structure in water (5-degrees-C, pH 5). Moreover, it was shown that the addition of increasing amounts of the polyanionic molecule heparin forced a displacement of the conformational equilibrium of those peptides toward higher proportions of the polyproline II structure. In contrast, when the temperature is raised such a structure gradually disappears, leading to more disordered conformations.