Use of a continuous flow dialysis apparatus to determine the effect of cations, buffers and osmotic pressure on ram spermatozoa.
Use of a continuous flow dialysis apparatus to determine the effect of cations, buffers and osmotic pressure on ram spermatozoa.
复制标题
使用连续流动透析装置测定阳离子、缓冲液和渗透压对公羊精子的影响。
DOI：
10.1530/jrf.0.0220253
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
J. Voglmayr
中科院分区：
文献类型：
--
作者：
P. Quinn;I. White;J. Voglmayr
Summary. When ram semen was dialysed against phosphate-glucose diluents with a cation composition similar to rete testis fluid, epididymal and ejaculated seminal plasma and ejaculated ram spermatozoa, the impedance change frequency (ICF) was maintained best over 6 hr in the latter diluent. Krebs-Ringer-glucose buffered with sodium phosphate or trisodium citrate maintained the ICF at a high level, whereas tris-HCl and veronal-HCl buffers caused a rapid decline in ICF. Hypertonic dialysis media severely depressed the ICF within 1 hr, whereas hypotonic diluents gave results similar to Krebs-Ringer-phosphate. Adding 1 mm-calcium or 5 to 51 mm-potassium to an isotonic dialysis fluid was beneficial and the effects on ICF were additive. The omission of potassium from the hypotonic diluent caused no significant reduction in ICF.
