Soil Nutrient Depletion Is Associated with the Presence of Burkholderia pseudomallei.
Soil Nutrient Depletion Is Associated with the Presence of Burkholderia pseudomallei.
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DOI：
10.1128/aem.02538-16
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发表时间：
2016-12-15
影响因子：
4.4
通讯作者：
Limmathurotsakul D
中科院分区：
文献类型：
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作者：
Hantrakun V;Rongkard P;Oyuchua M;Amornchai P;Lim C;Wuthiekanun V;Day NP;Peacock SJ;Limmathurotsakul D
假鼻疽伯克霍尔德氏菌是一种生活在土壤中的细菌，也是类鼻疽病的原因，据估计，全世界每年有89,000人死于类鼻疽病。由于反复接触这种细菌，农业工人感染的风险很高。与有机体的存在或不存在有关的土壤物理化学性质知之甚少。在这里，我们评估了从泰国61个稻田采集的6100个土壤样本中的土壤理化性质和假鼻疽杆菌的存在情况。假鼻疽杆菌的存在与粘土比例、水分比例、盐度水平、有机质百分比、镉的存在以及营养水平(磷、钾、钙、镁和铁)呈负相关。铜绿假单胞菌的存在与土壤酸度无关(P=0.54)。在多变量Logistic回归模型中，假鼻疽杆菌的存在与土壤有机质百分比(优势比[OR]0.06；95%可信区间[CI]，0.01~0.47；P=0.007)、盐度水平(OR，0.06；95%CI，0.01~0.74；P=0.03)和土壤水分百分比(OR，0.81；95%CI，0.66~1.00；P=0.05)呈负相关。我们的研究表明，假鼻疽杆菌在养分枯竭的稻田中茁壮成长。一些农业做法导致土壤养分下降，这可能会影响受影响地区假单胞菌的存在和数量。摘要假鼻疽伯克霍尔德氏菌是一种环境革兰氏阴性杆菌，是类鼻疽的病原菌。人类在皮肤接种、吸入或摄入环境中的细菌后患上这种疾病。假鼻疽杆菌在土壤中的存在定义了人和牲畜处于类鼻疽病风险的地理区域，但人们对与该生物存在相关的土壤性质知之甚少。我们评估了泰国东部、中部和东北部61个稻田的土壤性质和假单胞菌的存在。我们证明，这种微生物更常见于有机质和营养水平较低的土壤，包括磷、钾、钙、镁和铁。我们还证明，收获后焚烧作物残渣会减少土壤养分，这并不少见。一些农业做法导致土壤养分下降，这可能会影响受影响地区假单胞菌的存在和数量。
Burkholderia pseudomallei is a soil-dwelling bacterium and the cause of melioidosis, which kills an estimated 89,000 people per year worldwide. Agricultural workers are at high risk of infection due to repeated exposure to the bacterium. Little is known about the soil physicochemical properties associated with the presence or absence of the organism. Here, we evaluated the soil physicochemical properties and presence of B. pseudomallei in 6,100 soil samples collected from 61 rice fields in Thailand. The presence of B. pseudomallei was negatively associated with the proportion of clay, proportion of moisture, level of salinity, percentage of organic matter, presence of cadmium, and nutrient levels (phosphorus, potassium, calcium, magnesium, and iron). The presence of B. pseudomallei was not associated with the level of soil acidity (P = 0.54). In a multivariable logistic regression model, the presence of B. pseudomallei was negatively associated with the percentage of organic matter (odds ratio [OR], 0.06; 95% confidence interval [CI], 0.01 to 0.47; P = 0.007), level of salinity (OR, 0.06; 95% CI, 0.01 to 0.74; P = 0.03), and percentage of soil moisture (OR, 0.81; 95% CI, 0.66 to 1.00; P = 0.05). Our study suggests that B. pseudomallei thrives in rice fields that are nutrient depleted. Some agricultural practices result in a decline in soil nutrients, which may impact the presence and amount of B. pseudomallei bacteria in affected areas. IMPORTANCE Burkholderia pseudomallei is an environmental Gram-negative bacillus and the cause of melioidosis. Humans acquire the disease following skin inoculation, inhalation, or ingestion of the bacterium in the environment. The presence of B. pseudomallei in soil defines geographic regions where humans and livestock are at risk of melioidosis, yet little is known about the soil properties associated with the presence of the organism. We evaluated the soil properties and presence of B. pseudomallei in 61 rice fields in East, Central, and Northeast Thailand. We demonstrated that the organism was more commonly found in soils with lower levels of organic matter and nutrients, including phosphorus, potassium, calcium, magnesium, and iron. We also demonstrated that crop residue burning after harvest, which can reduce soil nutrients, was not uncommon. Some agricultural practices result in a decline in soil nutrients, which may impact the presence and amount of B. pseudomallei bacteria in affected areas.