Analysis of Plant Root Gravitropism
Analysis of Plant Root Gravitropism
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植物根系向地性分析
DOI：
10.1007/978-1-0716-2297-1_1
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发表时间：
2022
影响因子：
--
通讯作者：
Gilroy, Simon
中科院分区：
文献类型：
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作者：
Barker, Richard J.;Johns, Sarah;Trane, Ralph;Gilroy, Simon
重力是影响植物发育的重要因素，具有向重力性，即植物器官对重力方向的定向生长反应，导致每种植物在地上和地下都有其特有的形态。尽管从概念上来说很容易遵循，但随着内部发育线索和环境影响的变化，监测植物的定向生长反应可能会变得复杂。在这个方案中，我们讨论了向重力性分析的方法，重点是对根反应的自动化分析。对于拟南芥，我们建议使用5-8天大的幼苗进行简单的90°旋转。如果定期拍摄图像，并在苗木发育和重力测定期间记录环境元数据，这些数据可以用来揭示分析不同阶段的定量动力学模式。使用分析根系参数并以RSML格式存储该数据的软件为提取大量根系参数以表征主根和一系列侧根表型的生长提供了可能性。
Gravity is a powerful element in shaping plant development, with gravitropism, the oriented growth response of plant organs to the direction of gravity, leading to each plant’s characteristic form both above and below ground. Despite being conceptually simple to follow, monitoring a plant’s directional growth responses can become complex as variation arises from both internal developmental cues as well as effects of the environment. In this protocol, we discuss approaches to gravitropism assays, focusing on automated analyses of root responses. ForArabidopsis, we recommend a simple 90° rotation using seedlings that are 5–8 days old. If images are taken at regular intervals and the environmental metadata is recorded during both seedling development and gravitropic assay, these data can be used to reveal quantitative kinetic patterns at distinct stages of the assay. The use of software that analyzes root system parameters and stores this data in the RSML format opens up the possibility for a host of root parameters to be extracted to characterize growth of the primary root and a range of lateral root phenotypes.