Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans.
Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans.
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DOI：
10.1093/nar/28.21.4232
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发表时间：
2000-11
影响因子：
14.9
通讯作者：
T. Takanami;Akiyuki Mori;Hideyuki Takahashi;A. Higashitani
中科院分区：
文献类型：
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作者：
T. Takanami;Akiyuki Mori;Hideyuki Takahashi;A. Higashitani
减数分裂细胞对DNA损伤剂的敏感性尚不清楚。我们已经证明，秀丽隐杆线虫生殖腺的减数分裂粗线核对x射线辐射有高度抗性，但对紫外线辐射没有，而受精后的早期胚胎细胞和完全成熟的卵母细胞则没有。Ce-rdh-1基因[RAD51， DMC1 (LIM15)，同源物1或Ce-rad-51]是线虫基因组中唯一的细菌reca样基因，在减数分裂细胞中强烈表达，是减数分裂重组所必需的基因。通过RNA干扰使Ce-rdh-1基因沉默后，减数分裂细胞比早期胚胎细胞对x射线辐射更敏感。这是首次报道减数分裂细胞由于参与减数分裂同源重组的酶的高水平表达而对DNA链断裂具有高度抗性。
Sensitivity of meiotic cells to DNA damaging agents is little understood. We have demonstrated that the meiotic pachytene nuclei in the Caenorhabditis elegans gonad are hyper-resistant to X-ray irradiation, but not to UV irradiation, whereas the early embryonic cells after fertilization and the full grown oocytes are not. The Ce-rdh-1 gene [RAD51, DMC1 (LIM15), homolog 1 or Ce-rad-51], which is essential for the meiotic recombination, is the only bacterial recA-like gene in the nematode genome, and is strongly expressed in the meiotic cells. Following silencing of the Ce-rdh-1 gene by RNA interference, the meiotic cells become more sensitive to X-ray irradiation than the early embryonic cells. This is the first report that meiotic cells are hyper-resistant to DNA strand breaks due to the high level of expression of the enzyme(s) involved in meiotic homologous recombination.