Genetic characterization and recombinant history of a novel HIV-1 circulating recombinant form (CRF101_01B) identified in Yunnan, China
Genetic characterization and recombinant history of a novel HIV-1 circulating recombinant form (CRF101_01B) identified in Yunnan, China
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中国云南发现的一种新型 HIV-1 循环重组形式 (CRF101_01B) 的遗传特征和重组历史。
DOI：
10.1016/j.meegid.2019.04.024
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发表时间：
2019-09-01
影响因子：
3.2
通讯作者：
Xia, Xueshan
中科院分区：
文献类型：
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作者：
Zhang, Chunyue;Feng, Yue;Xia, Xueshan
在中国云南地区，B、C和CRF01_AE之间的HIV-1多重重组事件发生频率较高。在这里，我们的特点是一种新的HIV-1循环重组形式（CRF）组成的CRF01_AE和亚型B（CRF101_01 B）从三个流行病学无关的个人。基于近全长基因组（NFLG）序列的系统发育分析显示，CRF101_01B形成了一个独特的单系簇，由100%的高bootstrap值支持，与所有已知的HIV-1 CRF远相关。CRF101_01 B具有CRF01_AE骨架，其中两个B片段分别插入gag和pol区域。亚区树分析显示CRF01_AE骨架和插入的亚型B片段来源于泰国家系。此外，我们的研究发现CRF101_01B起源于1996-1998年左右。这一发现描述了一种新的HIV-1 CRF，并强调了在中国云南持续监测HIV-1毒株遗传多样性和复杂性的重要性。
The high frequency of multiple HIV-1 recombinant events among the B, C and CRF01_AE were constantly occurring in Yunnan China. Here, we characterized a novel HIV-1 circulating recombinant form (CRF) consisting of CRF01_AE and subtype B (CRF101_01B) from three epidemiologically unlinked individuals. Phylogenetic analysis based on near full length genome (NFLG) sequences revealed that CRF101_01B formed a distinct monophyletic cluster supported by a high bootstrap value of 100%, distantly related to all known HIV-1 CRFs. CRF101_01B had a CRF01_AE backbone with two B segments inserted, respectively, in the gag and pol region. Further, subregion tree analysis showed that CRF01_AE backbone and subtype B segment inserted originated from a Thailand lineage. In addition, our study found that CRF101_01B originated around the year 1996-1998. This findings described a novel HIV-1 CRF, and highlighted the importance of continual monitoring of genetic diversity and complexity of HIV-1 strains in Yunnan, China.