CHARACTERIZATION OF SEWAGE SOLID-ASSOCIATED VIRUSES AND BEHAVIOR IN NATURAL-WATERS
CHARACTERIZATION OF SEWAGE SOLID-ASSOCIATED VIRUSES AND BEHAVIOR IN NATURAL-WATERS
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DOI：
10.1016/0043-1354(78)90031-3
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发表时间：
1978-01-01
期刊：
影响因子：
12.8
通讯作者：
ABADIE, MG
中科院分区：
文献类型：
--
作者：
GERBA, CP;STAGG, CH;ABADIE, MG
噬菌体和动物病毒与固体的结合被证明具有保护作用，从而提高了在自然水域中的存活率和对氯灭活的抵抗力。试图通过将污水固体滞留在用胎牛血清处理的膜过滤器上来区分二级处理污水中的固体相关病毒和自由悬浮病毒，以防止自由悬浮病毒的吸附。用pH 11.5，0.05M甘氨酸缓冲液洗脱膜滤膜上的固体相关病毒。在二级处理后的污水排放中，与固体有关的总大肠杆菌和动物病毒的百分比分别为1.0%~24%和3%~100%。最大数量的固体结合噬菌体附着在大小大于8.0微米和小于0.65微米的颗粒上。自来水、湖水和河口水都能洗脱固相结合的大肠杆菌噬菌体。海水中大肠杆菌噬菌体的洗脱似乎与海水的盐度有关。从海水中的污水固体中洗脱出来的菌体可以重新吸附到自然产生的海洋沉积物中。
The association of bacteriophages and animal viruses with solids was demonstrated to have a protective effect, resulting in enhanced survival in natural waters and resistance to inactivation by Cl. Attempts were made to differentiate solid-associated viruses and freely suspended viruses in secondarily treated sewage by the retention of sewage solids on membrane filters treated with fetal calf serum to prevent adsorption of freely suspended virus. Solid-associated viruses collected on membrane filters were eluted with pH 11.5, 0.05 M glycine buffer. The percentage of the total coliphage and animal virus associated with solids in secondarily treated sewage discharges ranged from < 1.0-24% and 3-100%, respectively. The largest quantity of solid-associated coliphage was attached to particles greater than 8.0 .mu.m and less than 0.65 .mu.m in size. Tapwater, lake water and estuarine water were all capable of eluting solid-associated coliphages. Elution of coliphages in marine water appeared to be related to the salinity of the water. Coliphages eluted from sewage solids in seawater could readsorb to naturally occurring marine sediment.