Synthesis of sialoglycoconjugates during dimethylsulfoxide-induced erythrodifferentiation of friend leukemia cells.
Synthesis of sialoglycoconjugates during dimethylsulfoxide-induced erythrodifferentiation of friend leukemia cells.
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二甲亚砜诱导的友白血病细胞红细胞分化过程中唾液酸糖缀合物的合成。
DOI：
10.1016/0304-4165(82)90327-0
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Sartorelli,AC
中科院分区：
文献类型：
--
作者：
Brown,AE;Schwartz,EL;Dreyer,RN;Sartorelli,AC
我们以前已经表明，红系分化的朋友小鼠白血病细胞的二甲基亚砜导致唾液酸含量和净表面负电荷的减少。通过测量唾液酸从N-乙酰甘露糖胺的合成及其在成熟过程中从唾液酸缀合物的分解代谢去除来检查负责唾液酸含量降低的机制。早在暴露于二甲基亚砜后12小时，唾液酸糖缀合物中N-[3 H]乙酰甘露糖胺的掺入就出现减少。在未处理和二甲基亚砜处理的细胞中，掺入唾液酸糖缀合物的放射性相对稳定，这意味着唾液酸残基的分解代谢去除不是分化红白血病细胞表面唾液酸含量降低的因素。此外，对照组和处理组细胞的唾液酸转移酶活性无差异。然而，在加入二甲亚砜后48 h，N-[3 H]乙酰甘露糖胺掺入N-乙酰神经氨酸、CMP-N-乙酰神经氨酸和暂时鉴定为N-乙酰甘露糖胺-6-磷酸的物质的放射性显著降低。分化红白血病细胞中唾液酸生物合成的减少反映在暴露于二甲亚砜的细胞的神经氨酸酶处理释放的放射性标记的唾液酸的量减少83%。这些发现与极性溶剂诱导白血病细胞分化为更成熟形式所引发的细胞老化现象一致。
We have previously shown that erythroid differentiation of Friend murine leukemia cells by dimethylsulfoxide results in a decrease in sialic acid content and net negative surface charge. The mechanism responsible for the decrease in sialic acid content was examined by measuring the synthesis of sialic acid from N-acetylmannosamine and its catabolic removal from sialoconjugates during the maturation process. A decrease in the incorporation of N-[3 H] acetylmannosamine into sialoglycoconjugates occured as early as 12 h after exposure to dimethyl-sulfoxide. Radioactivity incorporated into sialoglycoconjugates was relatively stable in untreated and dimethylsulfoxide-treated cells, implying that catabolic removal of sialic acid residues was not a factor in the decreased surface sialic acid content of differentiated erythroleukemia cells. In addition, no difference existed between control and treated cells in sialyltransferase activity. Significant decreases occurred, however, in the incorporation of radioactivity from N-[3 H] acetylmannosamine into N-acetylneuraminic acid, CMP-N-acetylneuraminic acid and a material tentatively identified as N-acetylmannosamine-6-phosphate, 48 h after the addition of dimethylsulfoxide. The decrease in sialic acid biosynthesis in differentiated erythroleukemia cells was reflected by an 83% decrease in the amount of radioactively-labeled sialic acid released by neuraminidase treatment of cells exposed to dimethylsulfoxide. These findings are consistent with a cellular aging phenomenon triggered by the polar solvent-induced differentiation of the leukemic cells into more mature forms.