Expression of trefoil peptides (TFF1, TFF2, and TFF3) in gastric carcinomas, intestinal metaplasia, and non-neoplastic gastric tissues
Expression of trefoil peptides (TFF1, TFF2, and TFF3) in gastric carcinomas, intestinal metaplasia, and non-neoplastic gastric tissues
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DOI：
10.1002/path.1147
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发表时间：
2002-08-01
影响因子：
7.3
通讯作者：
Sung, JJY
中科院分区：
文献类型：
--
作者：
Leung, WK;Yu, J;Sung, JJY
三叶因子家族（TFF）结构域肽由三个成员组成，在肠粘膜防御和修复以及肿瘤发生中发挥作用。三个TFF成员在胃癌级联反应中的作用仍然不清楚。本研究检测了7个胃癌细胞系、50例胃癌及其癌旁组织和40例非癌患者的组织，以阐明TFF在胃癌发生的各个阶段的表达时序。通过RT-PCR、免疫组织化学和western blot检测TIFF的表达。在胃细胞系中经常检测到TFF 1、TFF 2和TFF 3的异常表达。具体而言，TFF 1在所有非癌症患者中检测到，但仅在50%的胃癌和66%的邻近正常组织中检测到。TFF 2在87.5%的非癌患者、34%的胃癌和58%的癌旁组织中表达。TFF 1和TFF 2在胃癌组织和癌旁组织中的表达有显著相关性（p < 0.001）。相比之下，25%的非癌症患者中检测到TFF 3，并且显示出对肠化生区域的偏好（p=0.005）。62%的胃癌和24%的邻近非癌组织显示TFF 3表达。在肠上皮化生的杯状细胞和肿瘤细胞的细胞质和细胞核中证明了对TFF 3的免疫反应性。TFF 1和TFF 2的进行性丢失以及TFF 3的诱导可能参与了多步骤胃癌发生途径的早期阶段。版权所有（C）2002约翰威利父子有限公司
Trefoil factor family (TFF) domain peptides consist of three members that play a role in intestinal mucosal defence and repair, and in tumourigenesis. The role of the three TFF members in the gastric carcinogenesis cascade remains poorly defined. This study examined seven gastric cell lines, 50 gastric cancers and their adjacent non-cancer tissues, and tissues from 40 non-cancer patients, in order to elucidate the chronology of TFF expression in various stages of gastric carcinogenesis. TIFF expression was determined by RT-PCR, immunohistochemistry, and western blot. Aberrant expression of TFF1, TFF2, and TFF3 was frequently detected in gastric cell lines. Specifically, TFF1 was detected in all non-cancer patients, but was detected in only 50% of gastric cancer and 66% of adjacent normal tissues. TFF2 expression was demonstrated in 87.5% of non-cancer patients, 34% of gastric carcinomas, and 58%, of adjacent non-cancer tissues. There was a significant correlation between TFF1 and TFF2 expression in gastric cancer and adjacent non-cancer tissues (p < 0.001). By contrast, TFF3 was detected in 25% of non-cancer patients and showed a predilection for areas with intestinal metaplasia (p=0.005). Sixty-two per cent of gastric cancers and 24% of neighbouring non-cancer tissues showed TFF3 expression. Immunoreactivity against TFF3 was demonstrated in goblet cells of intestinal metaplasia and within the cytoplasm and nuclei of tumour cells. Progressive loss of TFF1 and TFF2, together with the induction of TFF3, is likely to be involved in the early stage of the multi-step gastric carcinogenesis pathway. Copyright (C) 2002 John Wiley Sons, Ltd.