Persephonella hydrogeniphila sp. nov., a novel thermophilic, hydrogen-oxidizing bacterium from a deep-sea hydrothermal vent chimney.
Persephonella hydrogeniphila sp. nov., a novel thermophilic, hydrogen-oxidizing bacterium from a deep-sea hydrothermal vent chimney.
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嗜氢佩尔塞芬氏菌 sp.
DOI：
10.1099/ijs.0.02505-0
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发表时间：
2003
影响因子：
2.8
通讯作者：
Y. Sako
中科院分区：
文献类型：
--
作者：
S. Nakagawa;K. Takai;K. Horikoshi;Y. Sako
从日本Izu-Bonin Arc水母海山1385 m深处深海热液喷口烟囱样品中分离到一株新的嗜热氢氧化菌29W(T)，在静态和搅拌培养条件下，其细胞分别为球菌(直径0.9-1.0微米)和直杆(2.3-2.7微米)。新的分离物是一种专性化学自养细菌，通过呼吸硝酸盐与H2还原生长，形成最终产物N2。极低的O2浓度(最佳0.6-0.8%，v/v)也被用作替代电子受体，而还原的硫化物不作为电子供体。在50~72.5℃(最适70℃，40min倍增时间)，pH 5.5~7.6(最适pH 7.2)，1.5%和5.0%NaC l(最适2.5%)的条件下，可以观察到硝酸盐存在下的缺氧氢氧化生长。基因组DNA中G+C含量为37.3mol%。基于16S rDNA序列的系统发育分析表明，该分离物是最近被描述的Persehonella属的成员，属于水生目的一个潜在的新科。根据新分离株的生理和分子生物学特性，将其命名为Persehonella heliPhila sp.11月是提议的。模式菌株为29W(T)株(=JCM 11663(T)=DSM 15103(T))。
A novel thermophilic, hydrogen-oxidizing bacterium, designated 29W(T), was isolated from a deep-sea hydrothermal vent chimney sample collected from the Suiyo Seamount in the Izu-Bonin Arc, Japan, at a depth of 1385 m. The cells were cocci (0.9-1.0 microm in diameter) and straight rods (2.3-2.7 microm long) under static and agitated culture conditions, respectively. The new isolate was an obligate chemolithoautotroph growing by respiratory nitrate reduction with H2, forming N2 as a final product. A very low concentration of O2 (optimum 0.6-0.8%, v/v) was also used as an alternative electron acceptor while reduced sulfur compounds did not serve as electron donors. Anoxic hydrogen-oxidizing growth with nitrate was observed between 50 and 72.5 degrees C (optimum 70 degrees C; 40 min doubling time), pH 5.5 and 7.6 (optimum pH 7.2), and in the presence of 1.5 and 5.0% NaCl (optimum 2.5%). The G + C content of the genomic DNA was 37.3 mol%. Phylogenetic analysis based on 16S rDNA sequences indicated that the isolate was a member of the recently described genus Persephonella in a potential new family within the order Aquificales. On the basis of the physiological and molecular properties of the new isolate, the name Persephonella hydrogeniphila sp. nov. is proposed. The type strain is strain 29W(T) (= JCM 11663(T) = DSM 15103(T)).