Co-localization of vasoactive intestinal polypeptide, gamma-aminobutyric acid and choline acetyltransferase in neocortical interneurons of the adult rat
Co-localization of vasoactive intestinal polypeptide, gamma-aminobutyric acid and choline acetyltransferase in neocortical interneurons of the adult rat
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DOI：
10.1016/s0006-8993(97)00218-7
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发表时间：
1997-05-23
期刊：
影响因子：
2.9
通讯作者：
Zilles, K
中科院分区：
文献类型：
--
作者：
Bayraktar, T;Staiger, JF;Zilles, K
血管活性肠肽（VIP）免疫反应的中间神经元是大鼠大脑皮层柱状组织模式的组成部分。应用敏感镜技术，研究了VIP与经典抑制性神经递质γ-氨基丁酸（GABA）和乙酰胆碱合成酶胆碱乙酰转移酶（ChAT）在新皮层神经元的共定位。此外，ChAT与GABA的共定位的频率被确定。在118 VIP免疫反应神经元，主要是从初级躯体感觉皮层的样本中，它被证明，几乎所有的人都揭示GABA的免疫反应，因此，是GABA能的。此外，34%的主要双极，VIP阳性神经元含有ChAT，因此，推测也是胆碱能的。VIP和ChAT的共定位根据皮质层而变化。最后，88%的60 ChAT免疫反应阳性神经元也免疫染色GABA。结论：大鼠新皮层中几乎所有VIP免疫反应神经元和大部分胆碱能神经元都是利用GABA的抑制性中间神经元的部分重叠亚群。
Interneurons immunoreactive for vasoactive intestinal polypeptide (VIP) are integral elements of columnar organization patterns in the rat cerebral cortex. By application of the sensitive mirror technique, the co-localization of VIP with the classical inhibitory neurotransmitter gamma-aminobutyric acid (GABA) and the acetylcholine-synthesizing enzyme, choline acetyltransferase (ChAT), was investigated in neocortical neurons. Furthermore, the frequency of co-localization of ChAT with GABA was determined. In a sample of 118 VIP-immunoreactive neurons, mostly from the primary somatosensory cortex, it was demonstrated that virtually all of them reveal immunoreactivity for GABA and, therefore, are to be GABAergic. Moreover, 34% of mostly bipolar, VIP-positive neurons contained ChAT and are, thus, supposedly cholinergic as well. Co-localization of VIP and ChAT varied according to cortical laminae. Finally, 88% of a total of 60 ChAT-immunoreactive neurons were also immunostained for GABA. It is concluded that almost all VIP-immunoreactive neurons and most of the cholinergic neurons in rat neocortex represent partly overlapping subpopulations of inhibitory interneurons utilizing GABA.