The winged-helix transcription factor Trident is expressed in cycling cells
The winged-helix transcription factor Trident is expressed in cycling cells
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DOI：
10.1093/nar/25.9.1715
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发表时间：
1997-05-01
影响因子：
14.9
通讯作者：
Clevers, H
中科院分区：
文献类型：
--
作者：
Korver, W;Roose, J;Clevers, H
我们描述了三叉戟，叉头/翼螺旋家族的新成员，从小鼠胸腺的克隆和表征。在小鼠胚胎中，该基因在所有组织中表达，而在成年小鼠中仅在胸腺中检测到表达。进一步的分析表明，Trident的表达与细胞周期密切相关，与细胞类型无关。[H-3]胸苷掺入的时间表明，mRNA和蛋白质表达强烈上调后进入S期的细胞周期。此外，该蛋白在M期被磷酸化。PCR介导的最佳结合位点的选择产生了类似于其他家庭成员的共识基序。这些结果将Trident鉴定为转录因子，其可能参与细胞周期特异性基因调控。
We describe the cloning and characterization of Trident, a novel member of the fork head/winged-helix family, from murine thymus. In the mouse embryo, the gene was expressed in all tissues, whereas in adult mice expression was only detected in the thymus. Further analysis revealed that Trident expression strictly correlated with cell cycling, independent of cell type. Timing of [H-3]thymidine incorporation showed that mRNA and protein expression were strongly upregulated upon entry into the S phase of the cell cycle. Moreover, the protein was phosphorylated in M phase. PCR-mediated selection of optimal binding sites yielded a consensus motif resembling that of other family members. These results identify Trident as a transcription factor, which is likely involved in cell cycle-specific gene regulation.