The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann-Pick C1-like 1 in Caco-2 cells
The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann-Pick C1-like 1 in Caco-2 cells
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DOI：
10.1017/s0007114509991991
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发表时间：
2010-02-28
影响因子：
3.6
通讯作者：
Zheng, Yongchen
中科院分区：
文献类型：
--
作者：
Huang, Ying;Zheng, Yongchen
血胆固醇升高是与动脉粥样硬化和CHID相关的重要危险因素。对微调胆固醇稳态的介质的研究已经认识到益生菌是潜在的有益的。在这里，我们提供了描述尼曼-皮克C1样1（NPCIL 1）的细菌调节的数据，当弱表达时，会导致肠道对胆固醇的吸收显着减少。益生菌Lactobacillus acidophilus ATCC 4356降低了NPCIL 1基因表达，并抑制了Caco-2细胞中胶束胆固醇的细胞摄取。由ATCC 4356分泌的可溶性效应分子被证明是NPCIL 1减少的原因。此外，ATCC 4356部分通过肝脏X受体（LXR）介导这种作用。NPCIL 1和LXR在胆固醇代谢中的作用强调了使用益生菌（如ATCC 4356）管理高胆固醇血症的基础。
Elevated blood cholesterol is an important risk factor associated with atherosclerosis and CHID. The search for mediators that fine tune cholesterol homeostasis has recognised probiotics as being potentially beneficial. Here, we present data describing bacterial regulation of Niemann-Pick Cl-like 1 (NPCIL1), which, when weakly expressed, results in a marked reduction in intestinal absorption of cholesterol. The probiotic Lactobacillus acidophilus ATCC 4356 reduced NPCIL1 gene expression and inhibited the cellular uptake of micellar cholesterol in Caco-2 cells. Soluble effector molecules secreted by ATCC 4356 were shown to be responsible for the decrease in NPCIL1. Furthermore, ATCC 4356 mediated this effect partly through the liver X receptors (LXR). The role of NPCIL1 and the LXR in cholesterol metabolism underscores the basis for the use of probiotics, such as ATCC 4356, in managing hypercholesterolaemia.