Cell cycle and cyclic AMP-dependent phosphorylation of plasma membrane proteins p14 and p24: defects in smooth surface transformed cells.
Cell cycle and cyclic AMP-dependent phosphorylation of plasma membrane proteins p14 and p24: defects in smooth surface transformed cells.
复制标题
质膜蛋白 p14 和 p24 的细胞周期和环 AMP 依赖性磷酸化：光滑表面转化细胞中的缺陷。
DOI：
10.1002/jcb.240230117
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发表时间：
1983
影响因子：
4
通讯作者：
Scott,RE
中科院分区：
文献类型：
--
作者：
Boman,BM;Zschunke,MA;Scott,RE
Two proteins which are localized to the cytoplasmic surface of the plasma membrane, p14 and p24, undergo cyclic AMP‐dependent phosphorylation in rapidly growing nontransformed murine embryo cells. In this cell system, growth arrest in the G1phase of the cell cycle induced by growth factor deprivation is associated with the reversible loss in ability to phosphorylate these substrates. By contrast Simian virus 40 and methylcholanthrene transformed cells show both defective G1growth control and defects in their ability to phosphorylate p14 and p24 under all tested growth conditions. These data suggested a correlation between defects in the physophorylation of p14 and p24 and defects in the ability of transformed cells to G1growth arrest. The results of the current studies by contrast show that 3T3 T proadipocytcs which have been transformed by the smooth surface tumorigenesis method show different characteristics. They retain the ability to G1growth arrest in serum‐deficient medium. They show cyclic AMP‐dependent phosphorylation of p14 and p24 during exponential growth. They do not, however, down regulate p14 and p24 phosphorylation in association with G1growth arrest. These observations suggest that neoplastic transformation is not necessarily associated with absolute defects in the ability to phosphorylate p14 and p24. Rather, the results of the current study suggest that the inability to modulate the cyclic AMP‐dependent phosphorylation of plasma membrane p14 and p24 proteins during the G1phase of the cell cycle may be more tightly associated with neoplastic transformation.
