Chaperone Properties of the Bacterial Periplasmic Substrate-binding Proteins*
Chaperone Properties of the Bacterial Periplasmic Substrate-binding Proteins*
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细菌周质底物结合蛋白的伴侣特性*
DOI：
--
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发表时间：
1997
影响因子：
4.8
通讯作者：
T. Caldas
中科院分区：
文献类型：
--
作者：
G. Richarme;T. Caldas
细菌周质底物结合蛋白是细菌主动跨膜转运和/或趋化过程中的起始受体。它们中的每一种都以高亲和力结合特定的底物（例如糖、氨基酸或离子）。对于转运，每个结合蛋白与由两个疏水蛋白和两个亲水ATP酶亚基组成的同源膜复合物相互作用。对于趋化性，结合蛋白与特异性膜趋化性受体相互作用。本文报道了大肠杆菌寡肽结合蛋白OppA、大肠杆菌麦芽糖结合蛋白MalE。大肠杆菌的半乳糖结合蛋白MglB和沙门氏菌的半乳糖结合蛋白MglB与未折叠和变性的蛋白质相互作用，例如参与应激后蛋白质折叠和蛋白质复性的分子伴侣。这些周质底物结合蛋白在尿素变性后促进柠檬酸合酶和α-葡萄糖苷酶的功能性折叠。它们在热休克条件下阻止柠檬酸合酶的聚集，并且它们与几种未折叠的蛋白质形成稳定的复合物，例如还原的羧甲基α-乳白蛋白和未折叠的牛胰蛋白酶抑制剂。这些分子伴侣样功能通过结合蛋白的配体和无配体形式显示，并且它们在比其周质浓度低10 - 100倍的结合蛋白浓度下发生。这些结果表明，细菌周质底物结合蛋白，除了其功能的运输和趋化性，可能涉及蛋白质折叠和保护压力的周质。
Bacterial periplasmic substrate-binding proteins are initial receptors in the process of active transport across cell membranes and/or chemotaxis. Each of them binds a specific substrate (e.g. sugar, amino acid, or ion) with high affinity. For transport, each binding protein interacts with a cognate membrane complex consisting of two hydrophobic proteins and two subunits of a hydrophilic ATPase. For chemotaxis, binding proteins interact with specific membrane chemotaxis receptors. We report, herewith, that the oligopeptide-binding protein OppA of Escherichia coli, the maltose-binding protein MalE of E. coli, and the galactose-binding protein MglB of Salmonella typhimuriuminteract with unfolded and denatured proteins, such as the molecular chaperones that are involved in protein folding and protein renaturation after stress. These periplasmic substrate-binding proteins promote the functional folding of citrate synthase and α-glucosidase after urea denaturation. They prevent the aggregation of citrate synthase under heat shock conditions, and they form stable complexes with several unfolded proteins, such as reduced carboxymethyl α-lactalbumin and unfolded bovine pancreatic trypsin inhibitor. These chaperone-like functions are displayed by both the liganded and ligand-free forms of binding proteins, and they occur at binding protein concentrations that are 10–100-fold lower than their periplasmic concentration. These results suggest that bacterial periplasmic substrate-binding proteins, in addition to their function in transport and chemotaxis, might be implicated in protein folding and protection from stress in the periplasm.
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DOI：
10.1016/s0021-9258(18)45398-7
发表时间：
1987-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Żylicz;D. Ang;Costa Georgopoulos
通讯作者：
M. Żylicz;D. Ang;Costa Georgopoulos
影响因子：
10.5
作者：
S. Hultgren;Staff an Normark;S. Abraham
通讯作者：
S. Hultgren;Staff an Normark;S. Abraham
内向外膜囊泡周质运输的重建。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ames,GF;Nikaido,K;Groarke,J;Petithory,J
通讯作者：
Petithory,J
大肠杆菌 dnaK 复制蛋白的纯化和特性。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Zylicz,M;Georgopoulos,C
通讯作者：
Georgopoulos,C
影响因子：
16.6
作者：
QUIOCHO, FA
通讯作者：
QUIOCHO, FA