Minimally invasive high-speed imaging of sarcomere contractile dynamics in mice and humans.
Minimally invasive high-speed imaging of sarcomere contractile dynamics in mice and humans.
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DOI：
10.1038/nature07104
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发表时间：
2008-08-07
期刊：
影响因子：
64.8
通讯作者：
Schnitzer, Mark J.
中科院分区：
文献类型：
--
作者：
Llewellyn, Michael E.;Barretto, Robert P. J.;Delp, Scott L.;Schnitzer, Mark J.
肌节是横纹肌的基本收缩单位。我们对肌节动力学的了解主要来自肌纤维的体外研究和对活体肌肉光学衍射图的分析。这两种方法都涉及高度侵入性的程序，都不允许检查活体对象的单个肌瘤。在这里，我们报告了使用微创光学显微内窥镜直接可视化单个肌节及其动态长度变化，以观察活体小鼠和人类肌纤维中产生的光的二次谐波频率。使用直径小至350µm的显微内窥镜，我们对被动和激活肌肉中的单个肌节进行了成像。我们的测量允许体内表征随着身体姿势的改变而发生的肌节长度变化，并可视化肌节长度在总长度测定中不明显的局部变化。高速数据采集能够以毫秒级的分辨率观察肌节收缩动力学。这些实验为体内成像研究指明了方向，表明肌节性能如何随身体条件和生理状态而变化，以及成像诊断如何揭示神经肌肉疾病如何影响收缩动力学。
Sarcomeres are the basic contractile units of striated muscle. Our knowledge about sarcomere dynamics has primarily come from in vitro studies of muscle fibres and analysis of optical diffraction patterns obtained from living muscles. Both approaches involve highly invasive procedures and neither allows examination of individual sarcomeres in live subjects. Here we report direct visualization of individual sarcomeres and their dynamical length variations using minimally invasive optical microendoscopy to observe second-harmonic frequencies of light generated in the muscle fibres of live mice and humans. Using microendoscopes as small as 350 µm in diameter, we imaged individual sarcomeres in both passive and activated muscle. Our measurements permit in vivo characterization of sarcomere length changes that occur with alterations in body posture and visualization of local variations in sarcomere length not apparent in aggregate length determinations. High-speed data acquisition enabled observation of sarcomere contractile dynamics with millisecond-scale resolution. These experiments point the way to in vivo imaging studies demonstrating how sarcomere performance varies with physical conditioning and physiological state, as well as imaging diagnostics revealing how neuromuscular diseases affect contractile dynamics.
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