MicroRNA-143 inhibits cell migration and invasion by targeting matrix metalloproteinase 13 in prostate cancer (Retracted article. See vol. 27, pg. 57, 2023)
MicroRNA-143 inhibits cell migration and invasion by targeting matrix metalloproteinase 13 in prostate cancer (Retracted article. See vol. 27, pg. 57, 2023)
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DOI：
10.3892/mmr.2013.1501
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发表时间：
2013-08-01
影响因子：
3.4
通讯作者：
Qu, Ping
中科院分区：
文献类型：
--
作者：
Wu, Deyao;Huang, Ping;Qu, Ping
MicroRNA-143（miR-143）先前已报道在特定类型的癌症中下调，包括结肠直肠癌、膀胱癌、口腔鳞状细胞癌、垂体癌、宫颈癌、鼻咽癌、淋巴瘤和前列腺癌。在本研究中，检测了miR-143对前列腺癌细胞迁移和侵袭的影响。在用miR-143转染后，在前列腺癌细胞系中进行miR-143表达、细胞迁移和侵袭测定、荧光素酶测定和蛋白质印迹分析。结果表明，miR-143抑制DU 145和PC-3细胞的细胞迁移和侵袭。此外，据我们所知，miR-143首次被报道直接靶向前列腺癌中的基质金属蛋白酶13（MMP-13）。本研究的结果表明，miR-143通过靶向MMP-13抑制前列腺癌细胞系中的细胞迁移和侵袭。这些结果表明，miR-143可能适用于开发新的分子标记物和治疗方法，以抑制前列腺癌的转移。
MicroRNA-143 (miR-143) has been previously reported to be downregulated in specific types of cancer, including colorectal, bladder, oral squamous cell, pituitary, cervical, nasopharyngeal, lymphoma and prostate cancer. In the present study, the effects of miR-143 on prostate cancer cell migration and invasion were examined. Following transfection with miR-143, miR-143 expression, cell migration and invasion assays, luciferase assay and western blot analysis were conducted in prostate cancer cell lines. The results indicated that miR-143 inhibits cell migration and invasion in DU145 and PC-3 cells. In addition, to the best of our knowledge, miR-143 was reported for the first time to directly target matrix metalloproteinase 13 (MMP-13) in prostate cancer. The results of the present study demonstrated that miR-143 suppresses cell migration and invasion by targeting MMP-13 in prostate cancer cell lines. These results indicated that miR-143 may be suitable for the development of novel molecular markers and therapeutic approaches to inhibit metastasis in prostate cancer.