Individually addressable multi-nanopores for single-molecule targeted operations.
Individually addressable multi-nanopores for single-molecule targeted operations.
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DOI：
10.1021/acs.nanolett.9b05307
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发表时间：
2020-02
期刊：
影响因子：
10.8
通讯作者：
Paolo Cadinu;Minkyung Kang;B. P. Nadappuram;A. Ivanov;J. Edel
中科院分区：
文献类型：
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作者：
Paolo Cadinu;Minkyung Kang;B. P. Nadappuram;A. Ivanov;J. Edel
分子输运的微调是单分子方法中普遍存在的问题。后者甚至在功能强大的单分子方法中也是显而易见的，例如纳米孔传感，在这些方法中，对更详细的生物分子特征的探索往往受到对纳米孔检测体积内单个分子的动力学控制不足的限制。在这项工作中，我们引入并表征了一种可重新配置的多纳米孔结构，该结构使更多的通道能够操纵纳米孔中DNA分子的动力学。我们证明了这种器件的制造过程是快速和高度可重复性的，它由四个相邻的、可单独寻址的纳米孔组成，位于石英纳米管的顶端。通过单独调节每个纳米孔上的电场，这些设备可以在几种独特的合作检测模式下运行，从而以高效率和更高的时间分辨率移动、传感和捕获DNA分子。
The fine-tuning of molecular transport is a ubiquitous problem of single-molecule methods. The latter is evident even in powerful single-molecule methods such as nanopore sensing, where the quest for resolving more detailed biomolecular features is often limited by insufficient control of the dynamics of individual molecules within the detection volume of the nanopore. In this work, we introduce and characterize a reconfigurable multi-nanopore architecture that enables additional channels to manipulate the dynamics of DNA molecules in a nanopore. We show that the fabrication process of this device, consisting of four adjacent, individually addressable nanopores located at the tip of a quartz nanopipette, is fast and highly reproducible. By individually tuning the electric field across each nanopore, these devices can operate in several unique cooperative detection modes that allow moving, sensing, and trapping DNA molecules with high efficiency and increased temporal resolution.