Equivalency of Nuclear Transfer‐Derived Embryonic Stem Cells to Those Derived from Fertilized Mouse Blastocysts
Equivalency of Nuclear Transfer‐Derived Embryonic Stem Cells to Those Derived from Fertilized Mouse Blastocysts
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DOI：
10.1634/stemcells.2005-0537
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发表时间：
2006-09
期刊：
影响因子：
5.2
通讯作者：
S. Wakayama;M. Jakt;Masako Suzuki;R. Araki;Takafusa Hikichi;S. Kishigami;H. Ohta;N. Van Thuan;E. Mizutani;Y. Sakaide;S. Senda;Satoshi Tanaka;Mitsuhiro Okada;M. Miyake;M. Abe;S. Nishikawa;K. Shiota;T. Wakayama
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文献类型：
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作者：
S. Wakayama;M. Jakt;Masako Suzuki;R. Araki;Takafusa Hikichi;S. Kishigami;H. Ohta;N. Van Thuan;E. Mizutani;Y. Sakaide;S. Senda;Satoshi Tanaka;Mitsuhiro Okada;M. Miyake;M. Abe;S. Nishikawa;K. Shiota;T. Wakayama
治疗性克隆，即利用核移植（NT）从囊胚中产生胚胎干细胞（ESCs），已在小鼠和牛身上成功证明。然而，如果NT - ESCs存在异常，例如与生殖克隆产生的后代相关的异常，其科学和医疗用途可能被证明是有限的。为了评估NT - ESC的特性，我们从几个小鼠品系的成年体细胞中建立了150多个NT - ESC系。在这里，我们证明这些NT - ESCs能够在体内分化成所有功能的胚胎组织。此外，在组织依赖性差异DNA甲基化区域、DNA微阵列谱和高覆盖基因表达谱中，它们的多能性标记表达与囊胚来源的ESCs相同。重要的是，NT手术没有对细胞核造成不可逆的损伤。NT - ESCs和ESCs的这些相似性表明，体细胞NT治疗性克隆小鼠可为临床前干细胞研究提供可靠的模型。
Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT‐ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT‐ESCs, we established more than 150 NT‐ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT‐ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst‐derived ESCs in terms of their expression of pluripotency markers in the presence of tissue‐dependent differentially DNA methylated regions, in DNA microarray profiles, and in high‐coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT‐ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.