CHOLESTEROL ENHANCES CATIONIC LIPOSOME-MEDIATED DNA TRANSFECTION OF HUMAN RESPIRATORY EPITHELIAL-CELLS
CHOLESTEROL ENHANCES CATIONIC LIPOSOME-MEDIATED DNA TRANSFECTION OF HUMAN RESPIRATORY EPITHELIAL-CELLS
复制标题
DOI：
10.1007/bf01200214
复制
发表时间：
1995-02-01
影响因子：
4
通讯作者：
MALONE, RW
中科院分区：
文献类型：
--
作者：
BENNETT, MJ;NANTZ, MH;MALONE, RW
阳离子脂质体转染剂可用于将多核苷酸转移到细胞内，并已被提出用于人类肺部的基因治疗。在阳离子脂类制剂中添加胆固醇的效果已经通过首先与不同量的二油酰磷脂酰磷脂酰乙醇胺(DOPE)和胆固醇配制成阳离子脂类N-[1-(2，3-dioleoyloxy)propyl-N-[1(2-hydroxy)ethyl]-N，N-二甲基碘化铵(DOI)来测试。研究发现，胆固醇可以增强呼吸道上皮细胞和小鼠成纤维细胞中的脂质介导的转染率。这些发现将促进包括分化上皮细胞单层在内的多种细胞类型的核酸转染，因此可能有助于检测各种细胞类型的基因调控和发展肺基因治疗。
Cationic liposome transfection reagents are useful for transferring polynucleotides into cells, and have been proposed for human pulmonary gene therapy. The effect of adding cholesterol to cationic lipid preparations has been tested by first formulating the cationic lipid N-[1-(2,3-dioleoyloxy)propyl-N-[1(2-hydroxy)ethyl]-N,N-dimethyl ammonium iodide (DORI) with varying amounts of dioleoylphosphatidylethanolamine (DOPE) and cholesterol. Cholesterol was found to enhance lipid-mediated transfection in both the respiratory epithelial cells and mouse fibroblasts. These findings will facilitate nucleic acid transfection of many cell types including differentiated epithelial cell monolayers, and therefore may be useful for examining gene regulation in various cell types and for developing pulmonary gene therapy.