Blocking transcription of the human rhodopsin gene by triplex-mediated DNA photocrosslinking.
Blocking transcription of the human rhodopsin gene by triplex-mediated DNA photocrosslinking.
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通过三链体介导的 DNA 光交联阻断人类视紫红质基因的转录。
DOI：
10.1093/nar/28.21.4283
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发表时间：
2000
影响因子：
14.9
通讯作者：
Wensel,TG
中科院分区：
文献类型：
--
作者：
Intody,Z;Perkins,BD;Wilson,JH;Wensel,TG
To explore the ability of triplex-forming oligodeoxyribonucleotides (TFOs) to inhibit genes responsible for dominant genetic disorders, we used two TFOs to block expression of the human rhodopsin gene, which encodes a G protein-coupled receptor involved in the blinding disorder autosomal dominant retinitis pigmentosa. Psoralen-modified TFOs and UVA irradiation were used to form photoadducts at two target sites in a plasmid expressing a rhodopsin–EGFP fusion, which was then transfected into HT1080 cells. Each TFO reduced rhodopsin–GFP expression by 70–80%, whereas treatment with both reduced expression by 90%. Expression levels of control genes on either the same plasmid or one co-transfected were not affected by the treatment. Mutations at one TFO target eliminated its effect on transcription, without diminishing inhibition by the other TFO. Northern blots indicated that TFO-directed psoralen photoadducts blocked progression of RNA polymerase, resulting in truncated transcripts. Inhibition of gene expression was not relieved over a 72 h period, suggesting that TFO-induced psoralen lesions are not repaired on this time scale. Irradiation of cells after transfection with plasmid and psoralen–TFOs produced photoadducts inside the cells and also inhibited expression of rhodopsin–EGFP. We conclude that directing DNA damage with psoralen–TFOs is an efficient and specific means for blocking transcription from the human rhodopsin gene.
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影响因子：
14.9
作者：
Thekkumkat Thomas;C. Faaland;Michael A. Gallo;Thresla Thomas
通讯作者：
Thekkumkat Thomas;C. Faaland;Michael A. Gallo;Thresla Thomas
DNA 三链体的形成选择性抑制人类 T 细胞中的粒细胞-巨噬细胞集落刺激因子基因表达*
DOI：
10.1074/jbc.271.24.14438
发表时间：
1996
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
M. Kochetkova;M. Frances Shannon
通讯作者：
M. Frances Shannon
通过人无细胞提取物优先修复补骨脂素损伤的质粒 DNA 中的交联切口与单加合物。
影响因子：
2.9
作者：
P. Calsou;E. Sage;E. Moustacchi;B. Salles
通讯作者：
B. Salles
肝癌细胞中三螺旋形成对基因表达的抑制 (*)
DOI：
10.1074/jbc.270.47.28402
发表时间：
1995
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
G. Tu;Q. Cao;Y. Israel
通讯作者：
Y. Israel
影响因子：
14.9
作者：
Frank M. Orson;Douglas W. Thomas;W. Michael McShan;D. Kessler;Michael E. Hogan
通讯作者：
Frank M. Orson;Douglas W. Thomas;W. Michael McShan;D. Kessler;Michael E. Hogan