Characterization of Campylobacter jejuni isolated from dogs and humans using flaA-SVR fragment sequencing in Ismailia, Egypt
Characterization of Campylobacter jejuni isolated from dogs and humans using flaA-SVR fragment sequencing in Ismailia, Egypt
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DOI：
10.1016/j.cimid.2021.101675
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发表时间：
2021-06-04
影响因子：
2
通讯作者：
Ezzat, Mahmoud
中科院分区：
文献类型：
--
作者：
Abdelmageed, Hend A.;Mandour, Ahmed S.;Ezzat, Mahmoud
导读：狗是空肠弯曲杆菌的无症状携带者。在过去的十年里，宠物狗的数量在埃及不断增加。目的：探讨c的发生频率。狗和人的空肠感染，相关毒力基因的分子分型，以及flaA-SVR基因的测序。方法：对152份未配对的狗（72例）和人（80例）粪便样本进行空肠梭菌和弯曲杆菌23S rRNA检测，检测致病性基因包括mapA基因、virB11、flaA、wlaN、iam、tetO和aadA基因。对代表性分离株进行了flaA-扩增子测序。结果：c。狗和人空肠阳性率分别为20.8%和31.2%，所有分离株均检测到23S rRNA和mapA基因阳性。空肠C.空肠harbor virB11和wlaN分别为20%和0%，iam分别为10%和20%，tetO和aadA1分别为40%和20%，flaA分别为40%和20%。flaA-SVR序列在人和狗分离株间具有较高的同源性（94.8%），但在氨基酸序列上存在18个替换，表明狗和人的空肠梭菌与人源和禽源分离株接近。结论：本研究证实了c的比较序列分析。狗和一些埃及人的空肠flaA-SVR片段，表明它们之间的同源率很高。结果表明，空肠梭菌宿主犬是一个令人担忧的公共卫生问题，需要采取有效的卫生措施来预防埃及社区空肠梭菌人畜共患病。
Introduction: Dogs are known as asymptomatic carriers forCampylobacter jejuni. The number of pet dogs is increasing in Egypt in the last decade. Objective: This study aimed to investigate the frequency ofC. jejuni infection in dogs and humans, molecular typing of associated virulence genes, and flaA-SVR gene using sequencing.Methodology: 152 unpaired fecal swabs from dogs (n = 72) and humans (80) were examined for the presence of C. jejuni and Campylobacter 23S rRNA, and the pathogenicity genes including mapA genes, virB11, flaA, wlaN, iam, tetO, and aadA genes. Sequencing of the flaA- amplicon was also performed for the representative isolates.Results: The isolation rate ofC. jejuni was 20.8 % and 31.2 %, respectively in dogs and humans, and all isolates were tested positive for 23S rRNA and mapA genes. C. jejuni harbor virB11 and wlaN (20 %, 0%), iam (10 %, 20 %), tetO and aadA1 (40 %, both), and flaA (40 %, 20 %) in human and dog strains, respectively. The flaA-SVR sequences revealed high identity between human and dog isolates (94.8 %), but revealed 18 substitutions in the amino acid sequence, and showed that the dog and human C. jejuni were close to strains isolated from human and poultry sources.Conclusion: this study demonstrated the comparative sequence analysis ofC. jejuni flaA-SVR fragment in dogs and some Egyptians, which indicated a high identity percentage between them. The results suggest that C. jejuni reservoirs dogs is an alarming public health concern and effective hygienic measures are necessary for householding pets to prevent C. jejuni zoonosis in Egypt's community.