Measurement of serum IgE antibodies against Japanese cedar pollen (Cryptomeria japonica) in Japanese monkeys (Macaca fuscata) with pollinosis.
Measurement of serum IgE antibodies against Japanese cedar pollen (Cryptomeria japonica) in Japanese monkeys (Macaca fuscata) with pollinosis.
复制标题
测定患有花粉病的日本猴 (Macaca fuscata) 中针对日本柳杉花粉 (Cryptomeria japonica) 的血清 IgE 抗体。
DOI：
10.1111/j.1600-0684.1992.tb00597.x
复制
发表时间：
1992
影响因子：
0.7
通讯作者：
K. Fujimoto
中科院分区：
文献类型：
--
作者：
M. Sakaguchi;S. Inouye;K. Imaoka;H. Miyazawa;M. Hashimoto;H. Nigi;S. Nakamura;S. Gotoh;M. Minezawa;K. Fujimoto
用荧光间接酶联免疫吸附试验（ELISA）检测了7例花粉症猕猴血清中柳杉（Cryptomeria japonica，CJ）花粉变应原IgE抗体。所有的猴子被发现有特异性IgE的粗花粉抗原。特异性IgE水平与Pharmacia CAP系统测定的水平具有良好的相关性。然后用两种纯化的变应原（Cry j I和Cry j II）通过ELISA测定IgE抗体。我们发现，5只猴子对两种过敏原都有特异性IgE，尽管另外两只只对Cry j I或Cry j II有IgE;猴子对两种主要过敏原的免疫反应性不同。
IgE antibodies against allergens of Japanese cedar (Cryptomeria japonica, CJ) pollen in the serum of seven Japanese monkeys (Macaca fuscata) with pollinosis were measured by fluorometric indirect enzyme-linked immunosorbent assay (ELISA). All of the monkeys were found to have specific IgE to the crude pollen antigen. The specific IgE levels were well correlated with those determined by the Pharmacia CAP system. IgE antibodies were then assayed with two kinds of purified allergens (Cry j I and Cry j II) by the ELISA. We found that five monkeys had specific IgE to both allergens, although the other two had IgE only to Cry j I or Cry j II; there is different immune responsiveness to the two major allergens in the monkeys.