Biodegradation process of alpha-TCP particles and new bone formation in a rabbit cranial defect model.
Biodegradation process of alpha-TCP particles and new bone formation in a rabbit cranial defect model.
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DOI：
10.1002/jbm.b.30540
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发表时间：
2006-11
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影响因子：
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通讯作者：
Hidemichi Kihara;M. Shiota;Y. Yamashita;S. Kasugai
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作者：
Hidemichi Kihara;M. Shiota;Y. Yamashita;S. Kasugai
本研究的目的是观察纯α - 磷酸三钙（α - TCP）颗粒的生物降解过程，并确定α - TCP作为骨缺损空间维持物的功效。我们使用了14只兔子，在每只兔子身上制备了两个颅骨缺损。一个缺损作为对照空置，而另一个用直径约300μm的α - TCP颗粒填充。在第1周、第4周和第8周处死动物。然后将颅骨要么包埋在石蜡中用于制备脱钙标本，要么包埋在聚酯树脂中用于制备未脱钙标本。对所有标本进行组织学和组织形态计量学评估。由于α - TCP的降解，在第1周颗粒中出现“网状结构”，在第8周在该结构中观察到新骨。在任何时间点，对照组和实验组之间的新骨量均无显著差异。然而，在实验组中，即使在缺损中心，α - TCP表面的新骨也很明显，而在对照组中纤维结缔组织占主导。这些结果表明，α - TCP是一种可降解的骨传导材料，当应用于骨缺损时能够作为骨再生的空间维持物。虽然实验组和阴性对照组之间的总骨形成没有显著差异，但颗粒的空间维持和骨传导特性可能在更长期的研究中导致更完整的骨形成。
The purpose of the present study was to observe the biodegradation process of pure alpha-tricalcium phosphate (alpha-TCP) particles and to determine the efficacy of alpha-TCP as a space maintainer in a bone defect. We used 14 rabbits and prepared two cranial bone defects in each rabbit. One defect was left empty as a control, whereas the other was filled with alpha-TCP particles about 300 mum in diameter. Animals were sacrificed at 1 week, 4 weeks, and 8 weeks. The cranial bone was then embedded either in paraffin wax for the preparation of decalcified specimens, or in polyester resin for the preparation of nondecalcified specimens. All specimens were evaluated histologically and histomorphometrically. As a consequence of the degradation of alpha-TCP, a "reticulate structure" appeared in the particles at 1 week and new bone was observed in this structure at 8 weeks. The amount of new bone between the control and experimental groups was not significantly different at any of the time points. However, in the experimental group, new bone at the surface of alpha-TCP was evident even in the center of the defect whereas fibrous connective tissue was dominant in the control group. These results indicate that alpha-TCP is a degradable osteoconductive material that is able to act as a space maintainer for bone regeneration when applied to a bone defect. While there was no significant difference in total bone formation between the experimental and negative control groups, the space-maintaining and osteoconductive properties of the particles may result in more complete bone formation in longer-term studies.