Raman and mechanical properties correlate at whole bone- and tissue-levels in a genetic mouse model
Raman and mechanical properties correlate at whole bone- and tissue-levels in a genetic mouse model
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DOI：
10.1016/j.jbiomech.2010.10.009
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发表时间：
2011-01-11
影响因子：
2.4
通讯作者：
Nyman, Jeffry S.
中科院分区：
文献类型：
--
作者：
Bi, Xiaohong;Patil, Chetan A.;Nyman, Jeffry S.
骨的抗骨折能力取决于组织各层次上主要成分的组成、方向和分布。因此，为了确定拉曼光谱（RS）在识别强骨或坚韧骨与弱骨或脆骨之间差异方面的相关性，我们研究了拉曼衍生特性是否可以解释整个骨骼和组织水平的生物力学特性的差异，并且独立于传统的矿化测量。我们从野生型小鼠和缺乏基质金属蛋白酶 2 的小鼠中采集了股骨，因为已知突变型小鼠的矿化减少。接下来，RS 直接从完整的骨干中量化成分特性，然后通过微型计算机断层扫描来量化矿化密度 (Ct.TMD)。然后测试这些特性与通过对相同股骨进行三点弯曲测试确定的生物力学特性之间的显着性相关性。将收获的胫骨嵌入塑料中，横向切片并抛光，以获得每个样本的平均拉曼特性，然后将其与每个样本的平均纳米压痕特性相关联。将 v(1) 磷酸盐除以脯氨酸峰强度可得出矿物质与胶原蛋白之比与生物力学特性（全骨模量、强度、屈服后偏转加上纳米压痕模量）之间最强的相关性。此外，v(1)磷酸盐/脯氨酸和Ct.TMD的线性组合提供了基因型之间强度差异的最佳解释，并且它本身就是脆性的最佳解释变量。拉曼与断裂阻力之间的因果关系需要研究，但拉曼有评估断裂风险的潜力。由爱思唯尔有限公司出版
The fracture resistance of bone arises from the composition, orientation, and distribution of the primary constituents at each hierarchical level of organization. Therefore, to establish the relevance of Raman spectroscopy (RS) in identifying differences between strong or tough bone and weak or brittle bone, we investigated whether Raman-derived properties could explain the variance in biomechanical properties at both the whole bone and the tissue-level, and do so independently of traditional measurements of mineralization. We harvested femurs from wild-type mice and mice lacking matrix metalloproteinase 2 because the mutant mice have a known reduction in mineralization. Next, RS quantified compositional properties directly from the intact diaphysis followed by micro-computed tomography to quantify mineralization density (Ct.TMD). Correlations were then tested for significance between these properties and the biomechanical properties as determined by the three-point bending test on the same femurs. Harvested tibia were embedded in plastic, sectioned transversely, and polished in order to acquire average Raman properties per specimen that were then correlated with average nanoindentation properties per specimen. Dividing the v(1) phosphate by the proline peak intensity provided the strongest correlation between the mineral-to-collagen ratio and the biomechanical properties (whole bone modulus, strength, and post-yield deflection plus nanoindentation modulus). Moreover, the linear combination of v(1) phosphate/proline and Ct.TMD provided the best explanation of the variance in strength between the genotypes, and it alone was the best explanatory variable for brittleness. Causal relationships between Raman and fracture resistance need to be investigated, but Raman has the potential to assess fracture risk. Published by Elsevier Ltd.