Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea
Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea
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DOI：
10.1007/s10482-010-9430-8
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发表时间：
2010-06-01
影响因子：
2.6
通讯作者：
Li, Xiang
中科院分区：
文献类型：
--
作者：
Sun, Wei;Dai, Shikun;Li, Xiang
本文采用经典培养与鉴定、16S rDNA文库构建、16S rDNA限制性片段长度多态性分析和系统发育分析等方法，对采自南海岛的海绵放线菌多样性进行了研究。用7种不同的培养基共分离到184株菌株，根据它们的16S rRNA基因序列，根据它们的形态特征选择了24个菌株进行系统发育分析。结果表明，分离到的24株细菌分属于6个属，分别为盐生孢属、戈登氏菌属、分枝杆菌属、诺卡氏菌属、红球菌属和链霉菌属。这是首次报道在南中国海绵中发现盐孢子虫。随后，利用RFLP技术从放线杆菌16S rDNA文库中筛选出26个rDNA克隆，用于测序和系统发育分析。已知的放线杆菌属包括分枝杆菌属、无枝杆菌属、节杆菌属、短杆菌属、微新月杆菌属、诺卡氏菌属、假单胞菌属和链霉菌属等8个属。本研究有助于我们了解中国南海绵中放线菌的多样性。
In this report, the diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve collected from a remote island of the South China Sea was investigated employing classical cultivation and characterization, 16S rDNA library construction, 16S rDNA-restriction fragment length polymorphism (rDNA-RFLP) and phylogenetic analysis. A total of 184 strains were isolated using seven different media and 24 isolates were selected according to their morphological characteristics for phylogenetic analysis on the basis of their 16S rRNA gene sequences. Results showed that the 24 isolates were assigned to six genera including Salinispora, Gordonia, Mycobacterium, Nocardia, Rhodococcus and Streptomyces. This is the first report that Salinispora is present in a marine sponge from the South China Sea. Subsequently, 26 rDNA clones were selected from 191 clones in an Actinobacteria-specific 16S rDNA library of the H. perleve sample, using the RFLP technique for sequencing and phylogenetic analysis. In total, 26 phylotypes were clustered in eight known genera of Actinobacteria including Mycobacterium, Amycolatopsis, Arthrobacter, Brevibacterium, Microlunatus, Nocardioides, Pseudonocardia and Streptomyces. This study contributes to our understanding of actinobacterial diversity in the marine sponge H. perleve from the South China Sea.