Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration
Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration
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纤维内硅化胶原支架调节单核细胞以促进细胞归巢、血管生成和骨再生。
DOI：
10.1016/j.biomaterials.2016.10.050
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发表时间：
2017
期刊：
影响因子：
14
通讯作者：
Chen Ji Hua
中科院分区：
文献类型：
--
作者：
Sun Jin Long;Jiao Kai;Niu Li Na;Jiao Yang;Song Qun;Shen Li Juan;Tay Franklin R;Chen Ji Hua
单核细胞的免疫调节功能越来越受到人们的认可。硅化胶原支架（SCS）是通过用纤维内无定形二氧化硅浸润胶原基质而产生的，具有成骨和血管生成潜力，是组织工程中很有前途的候选材料。在这里，我们证明 SCS 通过单核细胞免疫调节促进原位骨再生和血管生成。在小鼠颅骨缺损模型中，可以从新骨形成区域中识别出数量增加的 TRAP 阳性单核细胞、巢蛋白阳性骨髓基质细胞 (BMSC) 以及 CD31 阳性和内粘蛋白阳性新血管。此外，SCS持续释放硅酸可刺激血源性单核细胞分化为TRAP阳性细胞，并增加SDF-1α、TGF-β1、VEGFa和PDGF-BB的表达。这些细胞因子进一步促进骨髓间充质干细胞和内皮祖细胞的归巢以及新血管形成。总而言之，这些新发现表明 SCS 具有增强祖细胞募集并通过单核细胞免疫调节促进骨生成和血管生成的能力。
The immunomodulatory functions of monocytes are increasingly being recognized. Silicified collagen scaffolds (SCSs), produced by infiltrating collagen matrices with intrafibrillar amorphous silica, exhibit osteogenic and angiogenic potential and are promising candidates in tissue engineering. Here, we demonstrate that SCS promotesin situbone regeneration and angiogenesis via monocyte immunomodulation. Increased numbers of TRAP-positive monocytes, nestin-positive bone marrow stromal cells (BMSCs) and CD31-positive and endomucin-positive new vessels can be identified from new bone formation regions in a murine calvarial defect model. In addition, sustained release of silicic acid by SCS stimulates differentiation of blood-derived monocytes into TRAP-positive cells, with increased expressions of SDF-1α, TGF-β1, VEGFa and PDGF-BB. These cytokines further promote homing of BMSCs and endothelial progenitor cells as well as neovascularization. Taken together, these novel findings indicate that SCSs possess the ability to enhance recruitment of progenitor cells and promote osteogenesis and angiogenesis by immunomodulation of monocytes.