The putative transforming protein of S13 avian erythroblastosis virus is a transmembrane glycoprotein with an associated protein kinase activity.
The putative transforming protein of S13 avian erythroblastosis virus is a transmembrane glycoprotein with an associated protein kinase activity.
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S13 禽成红细胞增多症病毒的推定转化蛋白是一种具有相关蛋白激酶活性的跨膜糖蛋白。
DOI：
10.1073/pnas.82.23.8237
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发表时间：
1985
影响因子：
11.1
通讯作者：
Beug,H
中科院分区：
文献类型：
--
作者：
Hayman,MJ;Kitchener,G;Vogt,PK;Beug,H
S13 是一种禽类逆转录病毒，可转化成纤维细胞和成红细胞。负责 S13 致癌作用的基因产物是 env 相关糖蛋白 gp155。在本报告中，我们表明 gp155 是一种具有 55 kDa 胞质结构域的跨膜蛋白。脉冲追踪分析表明，gp155 在翻译后被切割成两种糖基化蛋白：gp85 和 gp70。此外，我们发现酪氨酸蛋白激酶活性仅与微粒体和免疫复合物中的 gp70 蛋白相关。
S13 is an avian retrovirus that transforms both fibroblasts and erythroblasts. The gene product responsible for the oncogenic effects of S13 is the env-related glycoprotein gp155. In this report we show that gp155 is a transmembrane protein with a 55-kDa cytoplasmic domain. Pulse-chase analysis shows that gp155 was cleaved posttranslationally into two glycosylated proteins, gp85 and gp70. In addition, we show that a tyrosine protein kinase activity is associated only with the gp70 protein in microsomes and in immune complexes.