Bone formation in transplants of human bone marrow stromal cells and hydroxyapatite-tricalcium phosphate: Prediction with quantitative CT in mice
Bone formation in transplants of human bone marrow stromal cells and hydroxyapatite-tricalcium phosphate: Prediction with quantitative CT in mice
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DOI：
10.1148/radiol.2302011529
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发表时间：
2004-02-01
期刊：
影响因子：
19.7
通讯作者：
Robey, PG
中科院分区：
文献类型：
--
作者：
Mankani, MH;Kuznetsov, SA;Robey, PG
目得：确定定量计算机断层扫描（CT）是否可用于估计基于羟基磷灰石-磷酸三钙（HA-TCP）的移植物中新骨形成的程度。材料和方法：通过攻击培养的人骨髓基质细胞来产生骨形成移植物等份的HA-TCP颗粒并放置在免疫功能低下小鼠的皮下口袋中。8周后，对移植物进行单独成像;每次扫描包括一个体模。获得每个移植物的总体骨矿物质密度（BMD）。然后对相同移植物的苏木精染色切片进行组织学检查，这是评估骨形成的参考标准。由三名独立的盲法观察员对每例移植物中的骨范围进行半定量评分，评分范围为0 - 4;通过对三名观察员评分取平均值计算每例移植物的骨评分。将BMD与每次移植的组织学确定的骨评分进行比较。统计学评价包括（a）计算经验性受试者工作特征曲线以确定最佳BMD阈值和（B）确定BMD和骨评分之间的关系，包括Pearson相关系数的推导。在骨形成明显的移植物中（骨评分大于或等于3），平均BMD为600 mg/cm（3）K2 HPO 4或更高，而在骨形成较差的移植物中（骨评分< 3），平均BMD低于600 mg/cm（3）K2 HPO 4（P < .001）。在移植与可观的骨形成，骨密度成正比的矿化基质存在于新bone.CONCLUSION的程度：使用定量CT提供了一个实用的方法，为非侵入性测定矿化骨髓基质细胞和HA-TCP移植的新骨形成。（（C））RSNA，2004年。
PURPOSE: To determine whether quantitative computed tomography (CT) can be used to estimate the extent of new bone formation in hydroxyapatite-tricalcium phosphate (HA-TCP)-based transplants.MATERIALS AND METHODS: Bone-forming transplants were generated by attacking cultured human bone marrow stromal cells to aliquots of HA-TCP particles and were placed in subcutaneous pockets in immunocompromised mice. After 8 weeks, the transplants were individually imaged; each scan included a phantom. Overall bone mineral density (BMD) of each transplant was obtained. Hematoxylineosin-stained sections of the same transplants were then examined histologically, which is the reference standard for assessing bone formation. The extent of bone in each transplant was scored on a semiquantitative scale ranging from 0 to 4 by three independent blinded observers; the bone score for each transplant was calculated by averaging the three observer scores. BMD was compared with the histologically determined bone score for each transplant. Statistical evaluations included (a) calculation of empiric receiver operating characteristic curves to determine optimum BMD thresholds and (b) determination of the relationship between BMD and bone score, including derivation of Pearson correlation coefficients.RESULTS: One hundred twenty transplants were evaluated. Average BMD of 600 mg/cm(3) K2HPO4 or more was noted in transplants with appreciable bone formation (bone score greater than or equal to 3), while average BMD of less than 600 mg/cm(3) K2HPO4 was seen in transplants with poor bone formation (bone score < 3) (P < .001). Among transplants with appreciable bone formation, the BMD was proportional to the extent of mineralized matrix present in the new bone.CONCLUSION: Use of quantitative CT offers a practical approach for the noninvasive determination of new bone formation in mineralizing bone marrow stromal cells and HA-TCP transplants. ((C))RSNA, 2004.