High branched-chain alpha-keto acid intake, branched-chain alpha-keto acid dehydrogenase activity, and plasma and brain amino acid and plasma keto acid concentrations in rats.
High branched-chain alpha-keto acid intake, branched-chain alpha-keto acid dehydrogenase activity, and plasma and brain amino acid and plasma keto acid concentrations in rats.
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DOI：
10.1093/ajcn/52.2.313
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发表时间：
1990-08
期刊：
影响因子：
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通讯作者：
P L Crowell;K. P. Block;J J Repa-J;N. Torres;M D Nawabi;M G Buse;A. E. Harper
中科院分区：
文献类型：
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作者：
P L Crowell;K. P. Block;J J Repa-J;N. Torres;M D Nawabi;M G Buse;A. E. Harper
向大鼠饲喂含有大量用于治疗肾脏疾病的单个支链α-酮酸（BCKAs）或BCKAs组合的饲料。当饮食中含有单一BCKA时，其在血浆中的浓度高，并且其相应的氨基酸在血浆和脑中的浓度高。对照组大鼠肝脏BCKA脱氢酶（BCKD）活性为42%。摄入含0.38 mol/kg α-酮异己酸（KIC）、α-酮-β-甲基戊酸（KMV）或α-酮异戊酸（KIV）的饲料导致肝脏BCKD完全活化。食用含有BCKAs组合的饮食使肝脏的基础BCKD活性增加两倍。喂食KIV饲料（2倍）、KIC饲料（3倍）和KMV饲料（15倍）后，肌肉BCKD被激活。肝脏和肌肉的总BCKD活性不受饮食处理的影响。通过饮食BCKA激活肝脏和肌肉BCKD与它们在体外抑制BCKD激酶的能力一致。
Diets containing high quantities of individual branched-chain alpha-keto acids (BCKAs) or a combination of BCKAs as used for treatment of renal disease were fed to rats. When the diet contained a single BCKA, its concentration was high in plasma and the concentration of its corresponding amino acid was high in plasma and brain. Liver BCKA dehydrogenase (BCKD) was 42% active in control rats. Consumption of diets containing 0.38 mol/kg diet of alpha-ketoisocaproate (KIC), alpha-keto-beta-methylvalerate (KMV), or alpha-ketoisovalerate (KIV) resulted in complete activation of liver BCKD. Consumption of the diet containing the combination of BCKAs increased basal BCKD activity of liver twofold. Muscle BCKD was activated after feeding the KIV diet (2-fold), the KIC diet (3-fold), and the KMV diet (15-fold). Total BCKD activity of liver and muscle was unaffected by dietary treatments. Activation of liver and muscle BCKD by dietary BCKA is consistent with their ability to inhibit BCKD kinase in vitro.