Wound closure and metabolic parameter variability in a db/db mouse model for diabetic ulcers.
Wound closure and metabolic parameter variability in a db/db mouse model for diabetic ulcers.
复制标题
糖尿病溃疡 db/db 小鼠模型中的伤口闭合和代谢参数变异性。
DOI：
10.1016/j.jss.2008.01.023
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Lustbader,JoyceW
中科院分区：
文献类型：
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作者：
Trousdale,RhondaK;Jacobs,Sharone';Simhaee,DavidA;Wu,JuneK;Lustbader,JoyceW
糖尿病足溃疡是非创伤性下肢截肢的主要原因。伤口愈合研究人员通常使用db/db小鼠作为糖尿病模型，而切除伤口与慢性足部溃疡密切相关。最近的临床试验确定了糖尿病患者血糖控制和心血管并发症之间的相关性。本研究的目的是确定糖尿病的严重程度是否与糖尿病db/db小鼠中观察到的伤口愈合不良和广泛的伤口闭合变异性有关。材料和方法将成年雌性C57 BLKS/J、db+/−和db/db小鼠麻醉，然后创建1.5 × 1.5 cm的全层切除伤口。在术后第1、5、7、10、14和21天（POD）测量伤口闭合。在研究过程中还测量了体重、空腹血糖和空腹胰岛素。到POD 21时，野生型和db+/−小鼠均表现出完全的伤口闭合。在db/db小鼠中，在POD 21时仍存在开放性伤口。伤口闭合百分比范围很广，从24%到81%，平均为55%。尽管糖尿病参数之间有很强的相关性，伤口闭合率和严重程度之间没有显着的相关性diabetes.CONCLUSIONSDiabetic db/db小鼠表现出明显的损伤愈合的切除伤口模型。个体小鼠伤口闭合的变异性与肥胖、高血糖症、高胰岛素血症或胰岛素抵抗的严重程度无关。对基本糖尿病参数的广泛评价未提供对db/db小鼠模型中伤口愈合过程的显著了解。
BACKGROUNDDiabetic foot ulcers are a major cause of nontraumatic lower extremity amputations. Wound-healing researchers commonly use db/db mice as a model for diabetes, while the excisional wound correlates well with chronic foot ulcers. Recent clinical trials identified a correlation between glycemic control and cardiovascular complications in diabetic patients. The purpose of this study was to determine if the severity of diabetes was related to poor wound healing and the broad wound closure variability observed in diabetic db/db mice.MATERIALS AND METHODSAdult female C57BLKS/J, db+/−, and db/db mice were anesthetized followed by creation of a 1.5 × 1.5 cm full-thickness excisional wound. Wound closure was measured on postoperative days (PODs) 1, 5, 7, 10, 14, and 21. Weight, fasting blood glucose, and fasting insulin were also measured during the study.RESULTSBy POD 21 both wild-type and db+/− mice demonstrated complete wound closure. In db/db mice open wounds were still present at POD 21. There was a broad range of percent wound closure from 24 to 81% with a mean of 55%. Despite strong correlations between diabetic parameters, there was no significant correlation between wound closure rate and severity of diabetes.CONCLUSIONSDiabetic db/db mice exhibit a significant impairment of healing in the excisional wound model. The variability of wound closure for individual mice did not correlate with severity of obesity, hyperglycemia, hyperinsulinemia, or insulin resistance. An extensive evaluation of basic diabetes parameters does not provide significant insight into the wound-healing process in the db/db mouse model.