BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder.
BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder.
复制标题
携带与双相情感障碍相关的 BDNF SNP 的淋巴母细胞系中的 BDNF 表达。
DOI：
10.1097/ypg.0b013e328353ae66
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发表时间：
2012
影响因子：
0.9
通讯作者：
BiGSConsortium
中科院分区：
文献类型：
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作者：
Gao,Yonglin;Galante,Mathew;El-Mallakh,James;NurnbergerJr,JohnI;Delamere,NicholasA;Lei,Zhenmin;El-Mallakh,RifS;BiGSConsortium
目的确定脑源性神经营养因子（BDNF）的单核苷酸多态性（SNPs）是否与双相情感障碍有关。方法淋巴母细胞样细胞系（n= 30）从携带0，1，或与双相情感障碍相关的两个脑源性神经营养因子SNP拷贝结果单独血清剥夺或血清剥夺与钠离子载体莫能菌素结合的促凋亡应激因子没有改变细胞内的proBDNF。莫能菌素处理增加了成熟脑源性神经营养因子（mBDNF）蛋白水平（P< 0.05）。结论rs12273363在双相情感障碍中的作用与其功能无关。
ObjectiveTo determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction.MethodsLymphoblastoid cell lines (n= 30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized.ResultsProapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P< 0.05). There were no differences related to the presence of SNP or copy number.Conclusionrs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.