Development of microsatellite markers in Kmeria septentrionalis (Magnoliaceae), an endangered Chinese tree.
Development of microsatellite markers in Kmeria septentrionalis (Magnoliaceae), an endangered Chinese tree.
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DOI：
10.3732/ajb.1100039
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发表时间：
2011-06
影响因子：
3
通讯作者：
Yanfang Lin;Liyan Zeng;Qi-Wei Zhang;Yaofang Wang;Shaoqing Tang
中科院分区：
文献类型：
--
作者：
Yanfang Lin;Liyan Zeng;Qi-Wei Zhang;Yaofang Wang;Shaoqing Tang
PREMISE OF THE STUDY Microsatellite markers were developed in an endangered plant, Kmeria septentrionalis, to investigate its population genetic structure, gene flow, and mating systems. METHODS AND RESULTS Using the combined biotin capture method, 14 microsatellite primer sets were isolated and characterized. All of these markers showed polymorphism, and the number of alleles per locus ranged from 3 to 15 across 60 individuals from two populations. The observed and expected heterozygosities ranged from 0.2692 to 0.9667 and from 0.3271 to 0.8881, respectively. CONCLUSIONS These markers will facilitate further studies on the genetic diversity and mating systems of K. septentrionalis.
