Ethylene-responsive miRNAs in roots of Medicago truncatula identified by high-throughput sequencing at whole genome level
Ethylene-responsive miRNAs in roots of Medicago truncatula identified by high-throughput sequencing at whole genome level
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通过全基因组水平高通量测序鉴定蒺藜苜蓿根中的乙烯响应 miRNA
DOI：
10.1016/j.plantsci.2011.11.007
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发表时间：
2012-03-01
期刊：
影响因子：
5.2
通讯作者：
Zhang, Wenhao
中科院分区：
文献类型：
--
作者：
Chen, Lei;Wang, Tianzuo;Zhang, Wenhao
乙烯是一种经典的植物激素，在植物生长发育中具有多种功能。根伸长对乙烯敏感，使得用乙烯和乙烯前体1-氨基环丙烷-1-羧酸（ACC）处理抑制根生长。MicroRNA作为一类内源性非编码小RNA，通过影响靶基因的表达，在植物生长发育和激素信号转导中发挥重要作用。然而，还没有详细的研究来评估microRNA在介导植物中乙烯依赖性生理过程中的作用。蒺藜苜蓿是豆科植物分子生物学研究中广泛应用的模式植物。本研究构建了两个从毛茛根中提取的小分子RNA文库。采用高通量测序对小RNA文库进行测序，获得了超过30 M的原始读段。我们对301个已知的miRNAs进行了注释，并在两个文库中鉴定了3个新的miRNAs。治疗M。在含有10 μ M ACC的蒺藜中，8种miRNAs的表达发生了变化。通过生物信息学方法预测乙烯应答miRNAs的靶点。乙烯响应的miRNA在乙烯诱导的根伸长抑制中的潜在作用进行了讨论。这些结果是有用的功能特性的miRNA介导乙烯依赖的生理过程中一般和根伸长，特别是。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Ethylene is one of the classical plant hormones with a diverse function in plant growth and development. Root elongation is sensitive to ethylene such that treatments with ethylene and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) inhibit root growth. MicroRNA as one type of endogenous, non-coding small RNAs, plays an important role in regulation of plant growth, development and hormonal signaling by affecting expression of target genes. However, there has been no detailed study to evaluate the role of microRNAs in mediation of ethylene-dependent physiological processes in plants. Medicago truncatula is a model plant widely used for investigation of molecular biology in legume species. In this study, we constructed two small RNA libraries from roots of M. truncatula treated with and without ACC. High-throughput sequencing was employed to sequence the small RNA libraries, and more than 30 M raw reads were obtained. We annotated 301 known miRNAs and identified 3 new miRNAs in the two libraries. Treatment of M. truncatula with 10 mu M ACC led to changes in expression of 8 miRNAs. The targets of the ethylene-responsive miRNAs were predicted by bioinformatic approach. The potential role of the ethylene-responsive miRNAs in the ethylene-induced inhibition of root elongation is discussed. These results are useful for functional characterization of miRNAs in mediation of ethylene-dependent physiological processes in general and root elongation in particular. (C) 2011 Elsevier Ireland Ltd. All rights reserved.