Spheroid formation and functional restoration of human fetal hepatocytes on poly-amino acid-coated dishes after serial proliferation
Spheroid formation and functional restoration of human fetal hepatocytes on poly-amino acid-coated dishes after serial proliferation
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连续增殖后聚氨基酸包被培养皿上人胎儿肝细胞的球体形成和功能恢复
DOI：
10.1023/b:cyto.0000009819.28689.42
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发表时间：
2003
期刊：
影响因子：
2.2
通讯作者：
R. Ueoka
中科院分区：
文献类型：
--
作者：
T. Matsushita;Kohji Nakano;Yasufumi Nishikura;Kenta Higuchi;Akifumi Kiyota;R. Ueoka
原代人胎儿肝细胞在单层培养中增殖直至第9代。在增殖过程中，细胞的形态从接种后的成纤维细胞样形状转变为汇合后的上皮样多边形形状。增殖与氨解毒能力的丧失有关，而氨解毒能力对于生物人工肝的功能至关重要。细胞在聚谷氨酸或聚天冬氨酸包被的聚苯乙烯培养皿上形成球体，该培养皿在中性pH下具有带负电荷的表面。然而，这些细胞并未在具有带正电表面的聚赖氨酸或聚精氨酸包被的培养皿上形成球状体，据报道，该表面适合形成成体肝细胞的球状体。球状培养细胞的细胞色素P450（CYP 1A1、CYP1A2）活性约为单层培养细胞的两倍。通过用2%二甲基亚砜处理，细胞的氨解毒活性在球状培养物中得以恢复。这些结果表明，人胎儿肝细胞形成球体的条件与成人肝细胞不同，使用聚谷氨酸或聚天冬氨酸涂层可以改善增殖的人胎儿肝细胞的球体培养。
Primary human fetal hepatocytes proliferated in monolayer culture up to the 9th passage. During proliferation, the cells changed their morphology from a fibroblast-like shape after inoculation to an epithelia-like polygonal shape after they reached confluence. The proliferation was associated with the loss of ammonia detoxification capacity, which is essential for the function of bioartificial liver. The cells formed spheroids on a poly-glutamic acid- or poly-aspartic acid-coated polystyrene dish that had a negatively charged surface at neutral pH. However, the cells did not form spheroids on a poly-lysine- or poly-arginine-coated dish that had a positively charged surface, which is reportedly suitable to form spheroids for adult hepatocytes. The activity of cytochrome P450 (CYP 1A1, CYP1A2) of the cells in spheroid culture was about twice as high as that of the cells in monolayer culture. The ammonia detoxification activity of the cells was restored in spheroid culture by treatment with 2% dimethylsulfoxide. These results suggest that the conditions for human fetal hepatocytes to form spheroids are different from that for adult hepatocytes, and the use of poly-glutamic acid or poly-aspartic acid coating may improve spheroid culture of proliferative human fetal hepatocytes.
丙磺舒和对乙酰氨基酚在新鲜分离的大鼠肝细胞中调节 3-叠氮基-3-脱氧胸苷分解代谢。
影响因子：
5.8
作者：
Cretton,EM;Sommadossi,JP
通讯作者：
Sommadossi,JP
生物人工肝治疗严重肝衰竭的临床经验。
影响因子：
9
作者：
Watanabe,FD;Mullon,CJ;Hewitt,WR;Arkadopoulos,N;Kahaku,E;Eguchi,S;Khalili,T;Arnaout,W;Shackleton,CR;Rozga,J;Solomon,B;Demetriou,AA
通讯作者：
Demetriou,AA
DOI：
10.1016/0165-7992(85)90052-1
发表时间：
1985-03
期刊：
Mutation research
影响因子：
--
作者：
R. Lubet;R. Nims;R. T. Mayer;J. W. Cameron;L. Schechtman
通讯作者：
R. Lubet;R. Nims;R. T. Mayer;J. W. Cameron;L. Schechtman