In vivo determination of collecting lymphatic vessel permeability to albumin: a role for lymphatics in exchange.
In vivo determination of collecting lymphatic vessel permeability to albumin: a role for lymphatics in exchange.
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体内测定集合淋巴管对白蛋白的通透性：淋巴管交换的作用。
DOI：
10.1113/jphysiol.2009.179622
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Huxley,VirginiaH
中科院分区：
文献类型：
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作者：
Scallan,JoshuaP;Huxley,VirginiaH
虽然淋巴血管系统是流体和溶质稳态的核心，但它如何完成这一任务还没有很好的定义。为了阐明基础液和溶质稳态的基本机制，我们评估了幼年雄性大鼠肠系膜收集淋巴管和小静脉对大鼠血清白蛋白（PRSAs）的通透性。使用最初用于毛细血管的定量微荧光技术，我们验证了一个假设，即由于小静脉和收集淋巴具有共同的胚胎起源，它们的pras不会有显着差异。支持我们的假设，收集淋巴prsa的中位数（3.5±1.0 × 10−7cm s−1，N= 22）与静脉prsa的中位数（4.0±1.0 × 10−7cm s−1，N= 8，P= 0.61）没有显著差异。收集淋巴管时，根据表观prsa与压力的关系，得到扩散渗透率（Pd= 2.5 × 10 - 7cm s - 1）。虽然所测得的prsa、pdd和估计的收集淋巴和小静脉的水力电导率相似，但对流耦合的贡献不同，因为小静脉相对于体内收集淋巴经历了更高的静水压力。综上所述，这些数据证明了收集淋巴管作为交换血管的能力，能够渗出溶质和过滤液体。因此，这些数据为淋巴结前淋巴管集中腔内蛋白的理论提供了实验支持。
While it is well established that the lymphatic vasculature is central to fluid and solute homeostasis, how it accomplishes this task is not well defined. To clarify the basic mechanisms underlying basal fluid and solute homeostasis, we assessed permeability to rat serum albumin (PRSAs) in mesenteric collecting lymphatic vessels and venules of juvenile male rats. Using the quantitative microfluorometric technique originally developed for blood capillaries, we tested the hypothesis that as a consequence of venules and collecting lymphatics sharing a common embryological origin, theirPRSAswould not differ significantly. Supporting our hypothesis, the median collecting lymphaticPRSAs(3.5 ± 1.0 × 10−7cm s−1,N= 22) did not differ significantly from the median venularPRSAs(4.0 ± 1.0 × 10−7cm s−1,N= 8,P= 0.61). For collecting lymphatics the diffusive permeability (Pd= 2.5 × 10−7cm s−1) was obtained from the relationship of apparentPRSAsand pressure. While the measuredPRSAs,Pdand estimated hydraulic conductivity of collecting lymphatics and venules were similar, the contribution of convective coupling differs as a result of the higher hydrostatic pressure experienced by venules relative to collecting lymphaticsin vivo. In summary, the data demonstrate the capacity for collecting lymphatics to act as exchange vessels, able to extravasate solute and filter fluid. As a consequence these data provide experimental support for the theory that prenodal lymphatic vessels concentrate intraluminal protein.