An efficient in vitro method for mass propagation of a woody ornamentalIxora coccinea L.
An efficient in vitro method for mass propagation of a woody ornamentalIxora coccinea L.
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DOI：
10.1007/bf01142326
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发表时间：
1997-05
影响因子：
6.2
通讯作者：
Prakash Lakshmanan;C. Lee;C. Goh
中科院分区：
文献类型：
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作者：
Prakash Lakshmanan;C. Lee;C. Goh
研究了影响龙船花组培苗建立、芽生长、增殖和生根的各种因素，一种木本灌木。茎段（去头、3节）是最适合丛生芽增殖的外植体，在含2.5 μMBA的木本植物培养基（WPM）上培养时，产生了多次分枝的腋芽，培养6周后平均每个外植体产生27个芽。激动素、2-iP、玉米素和噻苯隆都能诱导丛生芽的形成，但效果不如BA。虽然增殖培养基中IAA的存在不利于芽增殖，但芽生长不受IAA的影响。大量愈伤组织的产生和试管苗的玻璃化是试管苗增殖过程中要避免的主要问题。在增殖培养基中加入TIBA显著减少了基底愈伤组织的形成，而用氟碳聚合物（三乙烯全氟烷基乙烯基醚）膜（Neoadhesive PFA膜）密封培养容器几乎完全消除了玻璃化。用AVG处理也实现了玻璃化苗数量的减少。根据使用BA补充的WPM和Neoadenosine膜的该方案，将有可能在1年内从单个茎切割产生超过100，000株植物。
Various factors that affect culture establishment, shoot growth, proliferation and rooting ofIxora coccineaL., a woody shrub, were studied. Stem cuttings (decapitated shoot, three nodes) were the most suitable explants for multiple-shoot proliferation, and when cultured on a woody plant medium (WPM) containing 2.5 μMBA produced axillary shoots which branched repeatedly, yielding an average of 27 shoots per explant after 6 weeks in culture. Kinetin, 2-iP, zeatin and thidiazuron all induced multiple-shoot formation, but were less effective than BA. While the presence of IAA in the multiplication medium was detrimental to shoot proliferation, shoot growth was not affected by IAA. The production of large amounts of basal callus and vitrification of shoots were the major problems to be avoided in proliferating shoot cultures. Addition of TIBA to the multiplication medium markedly reduced basal callusing, while sealing the culture vessels with a fluorocarbon polymer (tetrafluoroethyleneperfluoroalkyl vinyl ether) film (Neoflon PFA film) almost completely eliminated vitrification. A reduction in the number of vitrified shoots was also achieved with AVG treatment. Following this protocol of using BA-supplemented WPM and Neoflon film, it would be possible to produce more than 100,000 plants from a single stem cutting in 1 year.