EFFICACY OF DIFFERENT ROSE-BENGAL AND COMPLEMENT-FIXATION ANTIGENS FOR THE DIAGNOSIS OF BRUCELLA-MELITENSIS INFECTION IN SHEEP AND GOATS
EFFICACY OF DIFFERENT ROSE-BENGAL AND COMPLEMENT-FIXATION ANTIGENS FOR THE DIAGNOSIS OF BRUCELLA-MELITENSIS INFECTION IN SHEEP AND GOATS
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DOI：
10.1136/vr.134.16.415
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发表时间：
1994-04-16
影响因子：
2.2
通讯作者：
CAU, C
中科院分区：
文献类型：
--
作者：
BLASCO, JM;GARINBASTUJI, B;CAU, C
15种不同的玫瑰红抗原在pH、细胞浓度和与国际标准抗流产布鲁氏菌血清的凝集方面表现出巨大差异，表明缺乏国际标准化。与补体结合试验相比，评估了其在不同流行病学条件下诊断培养阳性绵羊、无布鲁氏菌母羊以及属于田间羊群的绵羊和山羊的羊种布鲁氏菌感染的敏感性和特异性。当测试无布鲁氏菌的绵羊或属于未接种布鲁氏菌病地区的羊群的动物时，所有玫瑰红抗原和补体结合试验都具有 100% 的特异性，但玫瑰红抗原与来自培养阳性绵羊或属于受感染羊群的动物的血清之间的敏感性存在很大差异。当使用最敏感的抗原时，感染了羊种 B 生物变种 1 或生物变种 3 的动物之间没有观察到玫瑰红敏感性差异。玫瑰红抗原的敏感性与细胞浓度之间的关系尚不清楚，但其敏感性与国际标准血清对抗原的标准化有关。在测试培养阳性的绵羊时，补体结合试验的敏感性低于孟加拉红试验。当使用根据欧盟规则标准化的抗原测试受感染鸡群动物的血清时，两种测试的敏感性没有观察到很大差异。然而，对于来自流行率较低的鸡群的动物的血清，观察到孟加拉红抗原的敏感性存在很大差异。此外，随着流行率的降低，两项测试中呈阳性的动物数量均减少，而仅其中一项测试中呈阳性的动物数量有所增加。
Fifteen different Rose Bengal antigens showed large differences with respect to pH, cell concentration and agglutination with the international standard anti-Brucella abortus serum, demonstrating the lack of international standardisation. Their sensitivity and specificity, compared with that of the complement fixation test, were evaluated for the diagnosis of B melitensis infection in culture-positive sheep, brucella-free ewes, and sheep and goats belonging to field flocks under different epidemiological conditions. All the Rose Bengal antigens and the complement fixation test had 100 per cent specificity when testing brucella-free sheep or animals belonging to flocks in unvaccinated brucellosis-free areas, but there mere large differences in sensitivity between the Rose Bengal antigens with sera from culture-positive sheep or from animals belonging to infected flocks. When using the most sensitive antigen, no difference was observed in Rose Bengal sensitivity between animals infected with either biovar 1 or biovar 3 of B melitensis. The relationship between the sensitivity of the Rose Bengal antigens and cell concentration was unclear, but their sensitivity was related to the standardisation of the antigens with the international standard serum. The complement fixation test was less sensitive than the Rose Bengal test when testing culture-positive sheep. When testing sera from animals belonging to infected flocks with antigens standardised according to European Union rules, no great differences were observed in the sensitivities of the two tests. However, great differences in sensitivity between the Rose Bengal antigens were observed with sera from animals belonging to flocks with low levels of prevalence, Moreover, the numbers of animals positive in both tests decreased, and the numbers of animals positive in only one of the tests increased, as the prevalence decreased.