Visualization of Plasma Membrane Compartmentalization by High-Speed Quantum Dot Tracking
Visualization of Plasma Membrane Compartmentalization by High-Speed Quantum Dot Tracking
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DOI：
10.1021/nl303151f
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发表时间：
2013-06-01
期刊：
影响因子：
10.8
通讯作者：
Lagerholm, B. Christoffer
中科院分区：
文献类型：
--
作者：
Clausen, Mathias P.;Lagerholm, B. Christoffer
在这项研究中，我们使用传统的宽视场显微镜在1.75 kHz的采样频率下具有高空间精度，对活细胞中用量子点标记的质膜分子进行了成像。许多得到的单分子轨迹足够长（多达几千步）以允许稳健的单轨迹分析。该分析表明，大多数研究的分子被短暂地限制在平均尺寸为（100-150 nm）（2）的纳米级隔室中，平均持续时间为50-100 ms。
In this study, we have imaged plasma membrane molecules labeled with quantum dots in live cells using a conventional wide-field microscope with high spatial precision at sampling frequencies of 1.75 kHz. Many of the resulting single molecule trajectories are sufficiently long (up to several thousand steps) to allow for robust single trajectory analysis. This analysis indicates that a majority of the investigated molecules are transiently confined in nanoscopic compartments with a mean size of (100-150 nm)(2) for a mean duration of 50-100 ms.