Elevation of interleukin-15 protein expression in bronchoalveolar fluid in acute lung allograft rejection
Elevation of interleukin-15 protein expression in bronchoalveolar fluid in acute lung allograft rejection
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DOI：
10.1378/chest.06-1257
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发表时间：
2007-02-01
期刊：
影响因子：
9.6
通讯作者：
Garrity, Edward R.
中科院分区：
文献类型：
--
作者：
Bhorade, Sangeeta M.;Yu, Andrew;Garrity, Edward R.
背景：急性排斥反应仍然是肺移植发病的主要来源。虽然白细胞介素（IL）-2已被牵连在急性排斥反应的主要T细胞生长因子，IL-2阻断不能完全防止急性排斥反应。最近，已发现IL-15（一种基质细胞衍生的细胞因子）与IL-2具有相似的生物学功能。我们推测，IL-15水平可能会升高，在急性肺排斥反应中存在的IL-2 blocket.Methods：急性同种异体移植排斥反应的42例肺移植受者中有21例。结果：肺移植受者急性排斥反应的BALF中IL-15平均水平（+/- SD）高于无排斥反应者（分别为25 +/- 25 pg/mL和4.5 +/- 1.5 pg/mL; p < 0.0001）。此外，肺移植急性排斥反应受者BALF中IL-15水平呈双峰分布。BALF中IL-2水平与急性排斥反应无关。BALF中IL-15水平与细菌、真菌或巨细胞病毒感染无关。结论：在IL-2受体阻断的情况下，BALF中IL-15水平升高，可能是肺移植急性排斥反应的重要介质。
Background: Acute rejection remains a major source of morbidity in lung transplantation. Although interleukin (IL)-2 has been the principal T-cell growth factor implicated in acute rejection, IL-2 blockade does not prevent acute rejection completely. Recently, IL-15, a stromal cell-derived cytokine, has been found to share a similar biological function with IL-2. We hypothesized that IL-15 levels may be elevated in acute lung rejection in the presence of IL-2 blockade.Methods: Acute allograft rejection developed in 21 of 42 lung transplant recipients. BAL fluid (BALF) was analyzed for IL-2 and IL-15 protein expression by standard enzyme-linked immunosorbent assay.Results: The average (+/- SD) BALF IL-15 level was higher in lung transplant recipients with acute rejection compared to those without rejection (25 +/- 25 pg/mL vs 4.5 +/- 1.5 pg/mL, respectively; p < 0.0001). In addition, there appeared to be a bimodal distribution of BALF IL-15 levels in lung transplant recipients with acute rejection. BALF IL-2 levels were not associated with acute rejection. BALF IL-15 levels were not associated with bacterial, fungal, or cytomegalovirus infection.Conclusion: These data show that BALF IL-15 levels are elevated in acute lung allograft rejection in the presence of IL-2 receptor blockade and may be an important mediator for acute rejection in lung transplantation.