Eye primordium transplantation in Xenopus embryo.
Eye primordium transplantation in Xenopus embryo.
复制标题
非洲爪蟾胚胎的眼原基移植。
DOI：
10.1007/bf00186787
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Graziadei,PP
中科院分区：
文献类型：
--
作者：
Koo,H;Graziadei,PP
从第23/24期的北方爪蟾移植了一部分眼原基，即假定的视网膜原基，以代替去除的嗅原基。这两个物种的细胞可以在荧光显微镜下区分，我们使用所得的嵌合体来确定移植的眼原基是否会抑制嗅觉原基的再生，是否会与其通常的目标，间脑连接，以及细胞迁移是否会发生从移植到宿主中枢神经系统或从宿主中枢神经系统移植。在所有的情况下，嗅原基迅速再生，和正常的嗅球发展。移植手术中眼柄的缺失导致视神经无法从发育中的视网膜发育出来。一个包含视神经轴突的细胞桥将移植的视网膜连接到间脑。来自移植物的细胞通过细胞桥自由迁移到几个CNS区域。它们的形态、拓扑结构、数量以及与其他宿主元素的关系与这些细胞属于神经胶质和神经元类型的假设是一致的。
A part of the eye primordium, the presumptive retinal anlage, was transplanted from stage-23/24Xenopus borealisto replace the removed olfactory anlage ofXenopus laevis. Cells of the two species can be distinguished under fluorescence microscopy, and we used the resulting chimeras to determine whether the transplanted eye primordium would inhibit the regeneration of the olfactory anlage, whether it would connect with its usual target, the diencephalon, and whether migration of cells would occur from the transplant to the host CNS or from the host CNS to the transplant. In all cases, the olfactory anlage regenerated promptly, and normal olfactory bulbs developed. Omission of the eye stalk in the transplant resulted in failure of an optic nerve to develop from the developing retina. A cellular bridge containing the optic axons connected the transplanted retina to the diencephalon. Cells from the transplant migrated freely through the cellular bridge to several CNS regions. Their morphology, topographic arrangement, number, and relations with other host elements are consistent with the hypothesis that these cells belong to both glia and neuron types.