Genotyping and quantitation of noroviruses in oysters from Two Distinct Sea areas in Japan
Genotyping and quantitation of noroviruses in oysters from Two Distinct Sea areas in Japan
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DOI：
10.1111/j.1348-0421.2007.tb03899.x
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发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Kimura, Hirokazu
中科院分区：
文献类型：
--
作者：
Nishida, Tomoko;Nishio, Osamu;Kimura, Hirokazu
诺如病毒（NV）是人类急性胃肠炎的病原体，包括牡蛎在内的贝类是该病毒的主要传播媒介。为了研究NVs的遗传特征，我们在2002年10月至2005年3月期间从两个不同的区域（A区：三陆海区；B区：濑户内海区）收集了1,512只牡蛎用于生食。我们检测到衣壳基因并对其进行系统发育分析。通过使用实时PCR进一步量化基因组拷贝数，在约5%的牡蛎中检测到NV capcid基因，并且它们表现出广泛的多样性。 B 区 2% 的牡蛎显示出相对较多的 NV，即/牡蛎有超过 100 个衣壳基因拷贝，而 A 区未观察到这种情况。当比较衣壳区域时，大多数从牡蛎和人类检测到的 NV 都具有遗传相关性。这些结果表明，从人类获得的 NV 和从牡蛎获得的 NV 表现出相互之间的潜在关系，并且日本牡蛎的一些种群积累了相对大量的 NV。
Norovirus (NV) is a causative agent of acute gastroenteritis in humans, and shellfishes including oysters act as major vehicles of the virus. To investigate the genetic characteristics of NVs, we collected 1,512 oysters for raw consumption between October 2002 and March 2005 from two distinct areas (area A: the Sanriku Sea area; area B: the Setouchi Sea area). We detected the capsid gene and subjected it to phylogenetic analysis. By further quantification of the copy number of the genome by using real-time PCR, the NV capcid gene was detected in approximately 5% of the oysters, and they showed wide diversity. Two percent of the oysters from area B showed relatively large number of NVs, i.e., over 100 copies of capsid gene/oyster, whereas this was not observed in area A. Most of the detected NVs from oysters and humans were genetically related when the capsid region was compared. These results suggested that NVs obtained from humans and those obtained from oysters showed a potential relationship to each other and that some populations of Japanese oysters accumulated a relatively large number of NVs.